PKC zeta Antibody (H-1) recommended for detection of PKC ζ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

PKC ζ Antibody (H-1): sc-17781

PKC ζ Antibody (H-1) is rated 4.8 out of 5 by 32.
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Datasheets

    • Anti-PKC ζ Antibody (H-1) is a mouse monoclonal IgG2a (kappa light chain) PKC ζ antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 573-592 at the C-terminus of PKC ζ of rat origin
    • Anti-PKC zeta Antibody (H-1) is recommended for detection of PKC ζ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; may cross-react with PKC ι and PKC λ
    • Anti-PKC zeta Antibody (H-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-17781 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PKC ζ (H-1): sc-17781.

does this antibody detect the phosphorylated form of apkc zeta?

Asked by: marti
Thank you for your question. PKC ζ Antibody (H-1): sc-17781 detects both phosphorylated and unphosphorylated PKC ζ.
Answered by: Tech Support Europe
Date published: 2019-09-02

does this antibody can analyse the expression of PKMzeta protein in brain tissue?

Asked by: mehar
Thank you for your question. Yes, we have used this mouse monoclonal antibody, PKC ζ (H-1): sc-17781 for immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue. This data is available on our website. This antibody is also very popular, and has been featured in over 30 publications. You can find a complete list of product citations for this antibody on our website, and you can search to see how others have used this antibody for IHC in brain tissue.
Answered by: Technical Support
Date published: 2017-05-12

What is the best secondary to use with PKC ζ Antibody (H-1): sc-17781 in Western Blot?

Asked by: jenni
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-09
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Rated 5 out of 5 by from 抗体很好用,效果比预期的要好 之前从公司要了试用的一小管抗体,回来后自己做免疫荧光实验,发现效果很好,准本直接从公司购买正式投入实验使用。
Date published: 2018-02-28
Rated 4 out of 5 by from Very good in IF Good apical signal in IF on mIMCD3 grown as spheroids in matrigel. Concentration 1/400.
Date published: 2017-11-07
Rated 5 out of 5 by from Excellent Antibody I used this to determine the PKC zeta levels expressed in melanoma cell lines MeWo and SK-MEL-2. WB worked really well with 1:2000 dilution, (4% BSA, 0.05% TBST).
Date published: 2017-10-24
Rated 5 out of 5 by from Great product We used this antibody for our western blot. give us good results.
Date published: 2017-09-27
Rated 5 out of 5 by from Expressions were much higher In the experiment, PKC ζ Antibody has good quality in Western blot, strong bands at 1:600 dilution, expressions were much higher.
Date published: 2017-09-04
Rated 5 out of 5 by from good staining This antibody works in staining of human kidney at 1:250 dilution. I am giving full stars as the staining is very stong and specific and antibody concentration needed very low.
Date published: 2017-08-19
Rated 4 out of 5 by from Detects band at correct size when Western blotting Western blot of PC3 cell total lysate provided a band at the correct size.
Date published: 2017-08-03
Rated 5 out of 5 by from WB analysis The product greatly reduced non-specific binding for WB analysis and enabled the analysis of mouse spleen tissue cells. We are satisfied with the reuslt and will continue using this product in the future.
Date published: 2017-07-22
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