PKC epsilon Antibody (E-5): sc-1681

PKC ε Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects PKC epsilon protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PKC ε Antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PKC epsilon protein plays a crucial role in cellular signaling pathways, particularly in the regulation of cell growth, differentiation, and survival. PKC epsilon protein is primarily located in the cytoplasm and can translocate to the plasma membrane upon activation, which is essential for function in mediating responses to growth factors and hormones. Such translocation allows PKC epsilon protein to interact with various substrates and signaling molecules, thereby influencing processes such as apoptosis and inflammation. PKC epsilon protein is involved in the modulation of gene expression and has been implicated in various diseases, including cancer and neurodegenerative disorders, making PKC epsilon protein a significant target for therapeutic interventions. Specific activation by diacylglycerols and phorbol esters further underscores PKC epsilon protein importance in cellular signaling and potential as a biomarker for disease states.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PKC epsilon Antibody (E-5) References:

1. A new member of the protein kinase C family, nPKC theta, predominantly expressed in skeletal muscle.  |  Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
2. Structural and functional diversities of a family of signal transducing protein kinases, protein kinase C family; two distinct classes of PKC, conventional cPKC and novel nPKC.  |  Ohno, S., et al. 1991. Adv Enzyme Regul. 31: 287-303. PMID: 1877391
3. Expression and characterization of protein kinase C-delta.  |  Olivier, AR. and Parker, PJ. 1991. Eur J Biochem. 200: 805-10. PMID: 1915352
4. HASF, a PKC-ε activator with novel features for cardiomyocyte protection.  |  Miura, T. 2014. J Mol Cell Cardiol. 69: 1-3. PMID: 24486196
5. Deconstructing the Role of PKC Epsilon in Glucose Homeostasis.  |  Schmitz-Peiffer, C. 2020. Trends Endocrinol Metab. 31: 344-356. PMID: 32305097
6. The roles of PKC-δ and PKC-ε in myocardial ischemia/reperfusion injury.  |  Chen, L., et al. 2021. Pharmacol Res. 170: 105716. PMID: 34102229
7. PKC-ε regulates vesicle delivery and focal exocytosis for efficient IgG-mediated phagocytosis.  |  D'Amico, AE., et al. 2021. J Cell Sci. 134: PMID: 34622926
8. Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids.  |  Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
9. Turnover of inositol phospholipids and signal transduction.  |  Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
10. The role of protein kinase C in cell surface signal transduction and tumour promotion.  |  Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
11. Protein kinase C as a possible receptor protein of tumor-promoting phorbol esters.  |  Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
12. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.  |  Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PKC epsilon Antibody (E-5) has 48 citations in a variety of scientific publications.

Primary antibodies like Anti-PKC epsilon Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PKC epsilon Antibody (E-5) Specifications

Alternate Product Name: PKC epsilon

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 723-737

Epitope Target: PKC ε

Epitope Species: human

Product Reactivity/Detection: PKC ε

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 48 citations in various scientific publications

Available Conjugates/Forms for PKC epsilon Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred PKC antibody is PKC Antibody (A-3): sc-17769.