Date published: 2025-11-17

1-800-457-3801

SCBT Portrait Logo
Seach Input

PKC Antibody (A-3): sc-17769

4.8(19)
Write a reviewAsk a question

Datasheets
  • PKC Antibody (A-3) is a mouse monoclonal IgG2a κ PKC antibody, cited in 53 publications, provided at 200 µg/ml
  • raised against amino acids 373-672 of PKC of human origin
  • PKC Antibody (A-3) is recommended for detection of PKC family members of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • Anti-PKC Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PKC Antibody (A-3) for WB and IHC(P) applications. These reagents are now offered in bundles with PKC Antibody (A-3) (see ordering information below).

QUICK LINKS

SEE ALSO...

PKC Antibody (A-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects PKC protein of mouse, rat, and human origin by WB, IP, IF, IHCP, and ELISA. PKC Antibody (A-3) is available in both non-conjugated and various conjugated forms, including agarose, HRP, PE, FITC, and multiple Alexa Fluor® conjugates. PKC monoclonal antibody (A-3) provides a reliable tool for researchers investigating the diverse roles of PKC in cellular signaling and implications in various pathological conditions. PKC proteins are primarily located in the cytoplasm and translocate to the cell membrane upon activation, which is vital for their role in signal transduction pathways. This translocation is essential for the activation of downstream signaling cascades that influence various physiological processes, including cell proliferation and apoptosis. The PKC family is composed of several isoforms, each exhibiting distinct tissue distribution and regulatory mechanisms, which underscores the importance of understanding their cellular localization and function in health and disease.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

PKC Antibody (A-3)

sc-17769
200 µg/ml
$316.00

PKC Antibody (A-3): m-IgG Fc BP-HRP Bundle

sc-528300
200 µg Ab; 10 µg BP
$354.00

PKC Antibody (A-3): m-IgGκ BP-HRP Bundle

sc-520643
200 µg Ab, 40 µg BP
$354.00

PKC Antibody (A-3): m-IgG2a BP-HRP Bundle

sc-547064
200 µg Ab; 10 µg BP
$354.00

PKC Antibody (A-3) AC

sc-17769 AC
500 µg/ml, 25% agarose
$416.00

PKC Antibody (A-3) HRP

sc-17769 HRP
200 µg/ml
$316.00

PKC Antibody (A-3) FITC

sc-17769 FITC
200 µg/ml
$330.00

PKC Antibody (A-3) PE

sc-17769 PE
200 µg/ml
$343.00

PKC Antibody (A-3) Alexa Fluor® 488

sc-17769 AF488
200 µg/ml
$357.00

PKC Antibody (A-3) Alexa Fluor® 546

sc-17769 AF546
200 µg/ml
$357.00

PKC Antibody (A-3) Alexa Fluor® 594

sc-17769 AF594
200 µg/ml
$357.00

PKC Antibody (A-3) Alexa Fluor® 647

sc-17769 AF647
200 µg/ml
$357.00

PKC Antibody (A-3) Alexa Fluor® 680

sc-17769 AF680
200 µg/ml
$357.00

PKC Antibody (A-3) Alexa Fluor® 790

sc-17769 AF790
200 µg/ml
$357.00

How many isoforms can this antibody detect exactly?

Asked by: fu800531
Thank you for your question. This antibody was raised against amino acids 373-672 of PKC alpha of human origin. The exact epitope has not been mapped. The expected molecular weight for this protein is ~80kDa.
Answered by: Tech Service
Date published: 2019-12-06

SC Support, We recently got some PKC Antibody (A-3): sc-17769 to try for free, thank you. I see that it was raised with amino acids 373-672 of PKC of human origin. We were wondering what isozymes of PKC will be recognized by this antibody? Thank you.

Asked by: Steve321
Thank you for your question. We have not determined the exact epitope recognized by this specific mouse monoclonal antibody, PKC (A-3): sc-17769. The antibody is raised against an antigen that shares significant sequence identity with PKC alpha, beta and gamma, and to a lesser extent, some identity with PKC epsilon, eta, theta and delta.
Answered by: Technical Support
Date published: 2017-07-31

How can PKC (A-3): sc-17769 monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Anna99
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. PKC (A-3): sc-17769 monoclonal antibody is currently available conjugated to phycoerythrin (sc-17769 PE), fluorescein (sc-17769 FITC), Alexa Fluor® 488 (sc-17769 AF488), and Alexa Fluor® 647 (sc-17769 AF647).
Answered by: Technical Support 14
Date published: 2023-08-11
  • y_2025, m_11, d_13, h_10CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_en_US, sid_17769, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 113ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from PKC antibodyThis is a good antibody. The antibody shows strong and specific signal in western blot.
Date published: 2023-08-06
Rated 5 out of 5 by from Great antibody for WBThis primary antibody worked very well in Western blot.
Date published: 2021-03-31
Rated 4 out of 5 by from good signalgood signal but with some background. Incubated overnight in 3% milk/PBST
Date published: 2021-03-17
Rated 5 out of 5 by from Great antibodyIt can be used to detect PKC for HaCaT cell in western blotting.
Date published: 2019-03-19
Rated 4 out of 5 by from Works well for IFI tried this antibody for early zebrafish embryos and it gave nice results.
Date published: 2017-10-30
Rated 5 out of 5 by from Great productwe used this antibody for our western blot. Give us a good result.
Date published: 2017-09-27
Rated 5 out of 5 by from PKC antibody "A-3"We got a free sample from Santa Cruz and wondered which isoform this antibody recognizes. Even though there is sequence homology between the antigen it was raised against and other isozymes, A-3 doesn't seem to recognize anything other than PKC-alpha at least among the ones we tried (see Western blot). Nice!
Date published: 2017-09-12
Rated 5 out of 5 by from good specificity in WBthis antibody shows strong and specific signal in WB
Date published: 2017-08-02
  • y_2025, m_11, d_13, h_10
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_19
  • loc_en_US, sid_17769, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 15ms
  • REVIEWS, PRODUCT
PKC Antibody (A-3) is rated 4.8 out of 5 by 19.
  • y_2025, m_11, d_13, h_10
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_19
  • loc_en_US, sid_17769, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 103ms
  • REVIEWS, PRODUCT