PIWIL2 Antibody (G-1): sc-377258

PIWIL2 Antibody (G-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects PIWIL2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PIWIL2 antibody (G-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PIWIL2, also known as HILI or cancer/testis antigen 80 (CT80), is a 973 amino acid protein that belongs to the argonaute family and features one PAZ domain and one PIWI domain. This cytoplasmic protein is predominantly expressed in adult testis and various tumors, playing a crucial role in regulating spermatogenesis and the production of primordial germ cells. PIWIL2 is involved in the meiotic differentiation of spermatocytes and the self-renewal of spermatogonial stem cells, which is vital for maintaining male fertility. PIWIL2 interacts with several key proteins that influence cellular processes, including PDGFR-β, which is associated with stem cell proliferation, and Glut1, which is involved in energy metabolism. PIWIL2 expression can affect cell-cell interactions through proteins such as Integrin α6, GJA7, THY-1, and CD9, as well as germ cell differentiation factors like STRA8. PIWIL2 may act as a regulatory factor in the Stat3/Bcl-xS/L/CCND1 signaling pathway, and PIWIL2 repression has been shown to inhibit tumor cell growth, highlighting a potential role as an oncogene by promoting proliferation and inhibiting apoptosis in cancerous cells.

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PIWIL2 Antibody (G-1) References:

1. Piwil2 inhibits keratin 8 degradation through promoting p38-induced phosphorylation to resist Fas-mediated apoptosis.  |  Jiang, S., et al. 2014. Mol Cell Biol. 34: 3928-38. PMID: 25113562
2. PIWIL2 suppresses Siah2-mediated degradation of HDAC3 and facilitates CK2α-mediated HDAC3 phosphorylation.  |  Zhang, Y., et al. 2018. Cell Death Dis. 9: 423. PMID: 29555935
3. PIWIL2 stabilizes β-catenin to promote cell cycle and proliferation in tumor cells.  |  Qiu, B., et al. 2019. Biochem Biophys Res Commun. 516: 819-824. PMID: 31262447
4. PIWIL2 may serve as a prognostic predictor in cancers: A systematic review and meta-analysis.  |  Hu, W., et al. 2020. J BUON. 25: 2721-2730. PMID: 33455119
5. PIWIL2 interacting with IKK to regulate autophagy and apoptosis in esophageal squamous cell carcinoma.  |  Zhao, X., et al. 2021. Cell Death Differ. 28: 1941-1954. PMID: 33469229
6. Attenuation of Piwil2 induced by hypoxic postconditioning prevents cerebral ischemic injury by inhibiting CREB2 promoter methylation.  |  Zhan, L., et al. 2023. Brain Pathol. 33: e13109. PMID: 35794855
7. PIWIL2 Regulates the Proliferation, Apoptosis and Colony Formation of Colorectal Cancer Cell Line.  |  Kishani Farahani, R., et al. 2023. Iran J Biotechnol. 21: e3176. PMID: 36811102
8. PIWIL2 restrains the progression of thyroid cancer via interaction with miR-146a-3p.  |  Lu, X., et al. 2023. BMC Endocr Disord. 23: 184. PMID: 37641092
9. Dexmedetomidine promotes colorectal cancer progression via Piwil2 signaling.  |  Dong, J., et al. 2024. Cell Oncol (Dordr). 47: 1459-1474. PMID: 38592610
10. PIWIL2/PDK1 Axis Promotes the Progression of Cervical Epithelial Lesions via Metabolic Reprogramming to Maintain Tumor-Initiating Cell Stemness.  |  Li, Y., et al. 2024. Adv Sci (Weinh). 11: e2410756. PMID: 39499767

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PIWIL2 Antibody (G-1) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-PIWIL2 Antibody (G-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PIWIL2 Antibody (G-1) Specifications

Alternate Product Name: PIWIL2

Product Clone Name: G-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 14-240

Epitope Target: PIWIL2

Epitope Species: mouse

Product Reactivity/Detection: PIWIL2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for PIWIL2 Antibody (G-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred PIWIL2 antibody is PIWIL2 Antibody (D-5): sc-377347.