PIWIL2 Antibody (D-5): sc-377347

PIWIL2 Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects PIWIL2 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PIWIL2 (D-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PIWIL2, also known as cancer/testis antigen 80 (CT80) and HILI, is a 973 amino acid member of the argonaute family, characterized by the presence of one PAZ domain and one PIWI domain. PIWIL2 is primarily located in the cytoplasm and is predominantly expressed in adult testis and various tumors, highlighting significance in reproductive biology and oncology. PIWIL2 plays a crucial role in regulating spermatogenesis and production of primordial germ cells, as well as in meiotic differentiation of spermatocytes and self-renewal of spermatogonial stem cells. PIWIL2 is involved in modulating expression of genes critical for stem cell proliferation, energy metabolism, cell-cell interactions, and germ cell differentiation, including PDGFR-β, Glut1, Integrin α6, GJA7, THY-1, CD9, and STRA8. PIWIL2 may act as a regulatory factor in Stat3/Bcl-xS/L/CCND1 signaling pathway, and PIWIL2 repression has been shown to inhibit tumor cell growth, indicating potential role as an oncogene by promoting proliferation and inhibiting apoptosis in cancerous cells.

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PIWIL2 Antibody (D-5) References:

1. Identification of eight members of the Argonaute family in the human genome.  |  Sasaki, T., et al. 2003. Genomics. 82: 323-30. PMID: 12906857
2. Mili, a mammalian member of piwi family gene, is essential for spermatogenesis.  |  Kuramochi-Miyagawa, S., et al. 2004. Development. 131: 839-49. PMID: 14736746
3. Stem cell protein Piwil2 modulates expression of murine spermatogonial stem cell expressed genes.  |  Lee, JH., et al. 2006. Mol Reprod Dev. 73: 173-9. PMID: 16261612
4. Stem-cell protein Piwil2 is widely expressed in tumors and inhibits apoptosis through activation of Stat3/Bcl-XL pathway.  |  Lee, JH., et al. 2006. Hum Mol Genet. 15: 201-11. PMID: 16377660
5. Derivation of male germ cells from bone marrow stem cells.  |  Nayernia, K., et al. 2006. Lab Invest. 86: 654-63. PMID: 16652109
6. A novel class of small RNAs bind to MILI protein in mouse testes.  |  Aravin, A., et al. 2006. Nature. 442: 203-7. PMID: 16751777
7. PIWIL2 promotes progression of non-small cell lung cancer by inducing CDK2 and Cyclin A expression.  |  Qu, X., et al. 2015. J Transl Med. 13: 301. PMID: 26373553
8. Co-expression of Piwil2/Piwil4 in nucleus indicates poor prognosis of hepatocellular carcinoma.  |  Zeng, G., et al. 2017. Oncotarget. 8: 4607-4617. PMID: 27894076
9. Expression of PIWIL2 in oral cancer and leukoplakia: Prognostic implications and insights from tumors.  |  Wang, S., et al. 2019. Cancer Biomark. 26: 11-20. PMID: 31322538
10. The Prognosis Value of PIWIL1 and PIWIL2 Expression in Pancreatic Cancer.  |  Li, W., et al. 2019. J Clin Med. 8: PMID: 31443431
11. Exosomes derived from Piwil2‑induced cancer stem cells transform fibroblasts into cancer‑associated fibroblasts.  |  Zhang, D., et al. 2020. Oncol Rep. 43: 1125-1132. PMID: 32323829
12. PIWIL2 is overexpressed in adenomyotic lesions of women with diffuse adenomyosis.  |  Mattia, MMC., et al. 2020. Arch Gynecol Obstet. 302: 925-933. PMID: 32613450

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PIWIL2 Antibody (D-5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-PIWIL2 Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PIWIL2 Antibody (D-5) Specifications

Alternate Product Name: Piwi Like RNA-Mediated Gene Silencing 2

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 157-191

Epitope Target: PIWIL2

Epitope Species: mouse

Product Reactivity/Detection: PIWIL2

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for PIWIL2 Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred PIWIL2 antibody is PIWIL2 Antibody (G-1): sc-377258.