PIWI Antibody (G-1): sc-390946

PIWI Antibody (G-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects PIWI protein of Drosophila melanogaster origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-PIWI antibody (G-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PIWI protein is a crucial component of the PIWI family, which is evolutionarily conserved and plays a significant role in stem cell self-renewal, gametogenesis, and RNA interference across organisms. PIWI protein is predominantly located in the nucleoplasm of both somatic and germline cells, where PIWI presence is vital for maintaining stem cell population balance. In the Drosophila ovary, PIWI protein is essential for asymmetric division of germline stem cells (GSCs), ensuring production and maintenance of daughter GSCs, although PIWI protein is not required for differentiation of committed daughter cells. The highly conserved C-terminal region of PIWI protein is critical for function, and removal from single GSCs significantly impairs division rate, indicating PIWI protein acts as a cell-autonomous promoter of GSC division. PIWI protein overexpression in somatic cells leads to increased GSC numbers and division rates, highlighting PIWI protein′s dual role as a stem cell dynamics regulator. PIWI (G-1) antibody serves as a valuable tool for studying stem cell regulation mechanisms and broader implications in developmental biology.

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PIWI Antibody (G-1) References:

1. piwi encodes a nucleoplasmic factor whose activity modulates the number and division rate of germline stem cells.  |  Cox, DN., et al. 2000. Development. 127: 503-14. PMID: 10631171
2. Two mouse piwi-related genes: miwi and mili.  |  Kuramochi-Miyagawa, S., et al. 2001. Mech Dev. 108: 121-33. PMID: 11578866
3. Molecular characterization of hiwi, a human member of the piwi gene family whose overexpression is correlated to seminomas.  |  Qiao, D., et al. 2002. Oncogene. 21: 3988-99. PMID: 12037681
4. RNAi is activated during Drosophila oocyte maturation in a manner dependent on aubergine and spindle-E.  |  Kennerdell, JR., et al. 2002. Genes Dev. 16: 1884-9. PMID: 12154120
5. PIWI-Interacting RNA: Its Biogenesis and Functions.  |  Iwasaki, YW., et al. 2015. Annu Rev Biochem. 84: 405-33. PMID: 25747396
6. PIWI Proteins and piRNAs in the Nervous System.  |  Kim, KW. 2019. Mol Cells. 42: 828-835. PMID: 31838836
7. PiWi RNA in Neurodevelopment and Neurodegenerative Disorders.  |  Chavda, V., et al. 2022. Curr Mol Pharmacol. 15: 517-531. PMID: 34212832
8. PIWI/piRNA-mediated regulation of signaling pathways in cell apoptosis.  |  Tan, Y., et al. 2022. Eur Rev Med Pharmacol Sci. 26: 5689-5697. PMID: 36066141
9. PIWI-interacting RNAs: Critical roles and therapeutic targets in cancer.  |  Zhou, J., et al. 2023. Cancer Lett. 562: 216189. PMID: 37076042
10. The burgeoning importance of PIWI-interacting RNAs in cancer progression.  |  Deng, X., et al. 2024. Sci China Life Sci. 67: 653-662. PMID: 38198029
11. PIWI-Interacting RNAs: A Pivotal Regulator in Neurological Development and Disease.  |  Pan, X., et al. 2024. Genes (Basel). 15: PMID: 38927589
12. A novel class of evolutionarily conserved genes defined by piwi are essential for stem cell self-renewal.  |  Cox, DN., et al. 1998. Genes Dev. 12: 3715-27. PMID: 9851978

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PIWI Antibody (G-1) has 4 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PIWI Antibody (G-1) Specifications

Alternate Product Name: PIWI

Product Clone Name: G-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-190

Epitope Target: PIWI

Epitope Species: Drosophila melanogaster

Product Reactivity/Detection: PIWI

Species Reactivity/Detection: d

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for PIWI Antibody (G-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin