Pit-1 Antibody (5E4): sc-47762

Pit-1 Antibody (5E4) is a mouse monoclonal IgG2a antibody that detects Pit-1 in mouse, rat, human, bovine, and porcine samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Pit-1, also known as growth hormone factor-1 (GHF-1), is a pivotal transcriptional regulator that plays a crucial role in the development of the anterior pituitary gland, where Pit-1 is essential for the differentiation and function of somatotropes, lactotropes, and thyrotropes. Pit-1 (5E4) antibody recognizes a bipartite DNA binding domain, which allows specific DNA sequence interactions to regulate gene expression. Notably, Pit-1 is expressed in two alternatively spliced forms, Pit-1a and Pit-1b, which exhibit distinct trans-activation capabilities, thereby influencing the production of key hormones such as growth hormone and prolactin. Mutations in the Pit-1 gene can lead to combined pituitary hormone deficiency (CPHD), affecting growth hormone, prolactin, and thyroid-stimulating hormone levels, which underscores its critical function in endocrine health. The gene encoding Pit-1 is located on human chromosome 3p11, highlighting its genetic significance in developmental biology and endocrinology. Anti-Pit-1 antibody (5E4) is an invaluable tool for researchers studying pituitary function and related disorders.

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Pit-1 Antibody (5E4) References:

1. Structures of cDNAs encoding chum salmon pituitary-specific transcription factor, Pit-1/GHF-1.  |  Ono, M. and Takayama, Y. 1992. Gene. 116: 275-9. PMID: 1634120
2. Vitamin D, Pit-1, GH, and PRL: possible roles in breast cancer development.  |  Perez-Fernandez, R., et al. 2007. Curr Med Chem. 14: 3051-8. PMID: 18220741
3. The pituitary-specific transcription factor GHF-1 is a homeobox-containing protein.  |  Bodner, M., et al. 1988. Cell. 55: 505-18. PMID: 2902927
4. Spironolactone dose‑dependently alleviates the calcification of aortic rings cultured in hyperphosphatemic medium with or without hyperglycemia by suppressing phenotypic transition of VSMCs through downregulation of Pit‑1.  |  Wang, P., et al. 2019. Mol Med Rep. 19: 3622-3632. PMID: 30896801
5. Pit-1 binding to specific DNA sites as a monomer or dimer determines gene-specific use of a tyrosine-dependent synergy domain.  |  Holloway, JM., et al. 1995. Genes Dev. 9: 1992-2006. PMID: 7649480
6. A novel pituitary transcription factor is produced by alternative splicing of the human GHF-1/PIT-1 gene.  |  Delhase, M., et al. 1995. Gene. 155: 273-5. PMID: 7721104
7. Functional interaction of c-Ets-1 and GHF-1/Pit-1 mediates Ras activation of pituitary-specific gene expression: mapping of the essential c-Ets-1 domain.  |  Bradford, AP., et al. 1995. Mol Cell Biol. 15: 2849-57. PMID: 7739565
8. The combination of Pit-1 and Pit-1T have a synergistic stimulatory effect on the thyrotropin beta-subunit promoter but not the growth hormone or prolactin promoters.  |  Haugen, BR., et al. 1994. Mol Endocrinol. 8: 1574-82. PMID: 7877626
9. Ligand-dependent, Pit-1/growth hormone factor-1 (GHF-1)-independent transcriptional stimulation of rat growth hormone gene expression by thyroid hormone receptors in vitro.  |  Suen, CS. and Chin, WW. 1993. Mol Cell Biol. 13: 1719-27. PMID: 8441408
10. Molecular cloning of gilthead seabream (Sparus aurata) pituitary transcription factor GHF-1/Pit-1.  |  Martínez-Barberá, JP., et al. 1997. Gene. 185: 87-93. PMID: 9034317

The preferred Pit-1 antibody is Pit-1 Antibody (G-2): sc-25258.