PIPK I β Antibody (F-4): sc-514169

PIPK I β Antibody (F-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects PIPK I beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PIPK I β (F-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The phosphatidylinositol-4-phosphate-5-kinase (PIPK) family plays a crucial role in synthesizing phosphatidylinositol-4,5-bisphosphate, a key lipid that regulates essential cellular processes such as cell proliferation, survival, membrane trafficking, and cytoskeletal organization. Within this family, PIPK I β, also known as STM7, is particularly important due to its specific role in phosphorylating phosphatidylinositol 4-phosphate at the 5-hydroxyl position, which is vital for the generation of signaling molecules that mediate various cellular responses. The PIPK family is categorized into three types—type I, type II, and type III—each with distinct substrate specificity and subcellular localization, determined by their unique activation loops. PIPK I β is expressed in brain tissue and interacts with other signaling proteins, contributing to the intricate network of cellular signaling pathways. Understanding the function and interactions of PIPK I β is essential for elucidating its role in health and disease, particularly in neurobiology and cancer research.

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PIPK I β Antibody (F-4) References:

1. The activation loop of phosphatidylinositol phosphate kinases determines signaling specificity.  |  Kunz, J., et al. 2000. Mol Cell. 5: 1-11. PMID: 10678164
2. Autophosphorylation of type I phosphatidylinositol phosphate kinase regulates its lipid kinase activity.  |  Itoh, T., et al. 2000. J Biol Chem. 275: 19389-94. PMID: 10777481
3. Phosphatidylinositol 4-phosphate 5-kinase is indispensable for mouse spermatogenesis.  |  Hasegawa, H., et al. 2012. Biol Reprod. 86: 136, 1-12. PMID: 22321832
4. Cis-silencing of PIP5K1B evidenced in Friedreich's ataxia patient cells results in cytoskeleton anomalies.  |  Bayot, A., et al. 2013. Hum Mol Genet. 22: 2894-904. PMID: 23552101
5. Distribution and localization of phosphatidylinositol 5-phosphate, 4-kinase alpha and beta in the brain.  |  Noch, EK., et al. 2021. J Comp Neurol. 529: 434-449. PMID: 32449185
6. PIP5K1C phosphoinositide kinase deficiency distinguishes PIKFYVE-dependent cancer cells from non-malignant cells.  |  Roy, A., et al. 2023. Autophagy. 19: 2464-2484. PMID: 36803256
7. LRRC8A as a central mediator promotes colon cancer metastasis by regulating PIP5K1B/PIP2 pathway.  |  Zhang, H., et al. 2024. Biochim Biophys Acta Mol Basis Dis. 1870: 167066. PMID: 38350542
8. The cloning and sequence of the C isoform of PtdIns4P 5-kinase.  |  Divecha, N., et al. 1995. Biochem J. 309 (Pt 3): 715-9. PMID: 7639683
9. Type I phosphatidylinositol-4-phosphate 5-kinases are distinct members of this novel lipid kinase family.  |  Loijens, JC. and Anderson, RA. 1996. J Biol Chem. 271: 32937-43. PMID: 8955136
10. Type I phosphatidylinositol-4-phosphate 5-kinases. Cloning of the third isoform and deletion/substitution analysis of members of this novel lipid kinase family.  |  Ishihara, H., et al. 1998. J Biol Chem. 273: 8741-8. PMID: 9535851
11. Type I phosphatidylinositol-4-phosphate 5-kinases synthesize the novel lipids phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 5-phosphate.  |  Tolias, KF., et al. 1998. J Biol Chem. 273: 18040-6. PMID: 9660759
12. Structure of type IIbeta phosphatidylinositol phosphate kinase: a protein kinase fold flattened for interfacial phosphorylation.  |  Rao, VD., et al. 1998. Cell. 94: 829-39. PMID: 9753329

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PIPK I β Antibody (F-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PIPK I β Antibody (F-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PIPK I β Antibody (F-4) Specifications

Alternate Product Name: PIPK I β

Product Clone Name: F-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 406-540

Epitope Target: PIPK I β

Epitope Species: human

Product Reactivity/Detection: PIPK I β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PIPK I β Antibody (F-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred PIPK I antibody is PIPK I Antibody (D-12): sc-365238.