PICOT Antibody (D-10): sc-390068
- PICOT Antibody (D-10) is a mouse monoclonal IgG1 κ PICOT antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 31-69 near the N-terminus of PICOT of human origin
- PICOT Antibody (D-10) is recommended for detection of PICOT of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PICOT Antibody (D-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PICOT Antibody (D-10) for WB applications. These reagents are now offered in bundles with PICOT Antibody (D-10) (see ordering information below).
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PICOT Antibody (D-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects PICOT protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PICOT (D-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PICOT protein, also known as GLRX3, GRX3, GRX4, GLRX4, HUSSY-22, TXNL2, or TXNL3, is a 335 amino acid protein characterized by one thioredoxin domain and two glutaredoxin domains. PICOT protein is primarily localized in the cytoplasm and cell cortex, where PICOT plays a crucial role in regulating the thioredoxin system, which is essential for maintaining cellular redox homeostasis. Notably, PICOT protein is involved in inhibiting cardiac hypertrophy, a condition characterized by the thickening of the heart muscle often due to high blood pressure, by negatively regulating nuclear factor of activated T-cells (NFAT) signaling. Although PICOT protein contains a thioredoxin domain, PICOT lacks the two redox-reactive cysteines necessary for catalytic activity, indicating that PICOT does not function as a traditional thioredoxin. PICOT expression is prominent in the testis, heart, and spleen, with lower levels found in the thymus, lung, colon, placenta, and small intestine, highlighting PICOT′s potential significance in various physiological processes and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PICOT Antibody (D-10) References:
- Inhibition of the c-Jun N-terminal kinase/AP-1 and NF-kappaB pathways by PICOT, a novel protein kinase C-interacting protein with a thioredoxin homology domain. | Witte, S., et al. 2000. J Biol Chem. 275: 1902-9. PMID: 10636891
- PICOT-HD: a highly conserved protein domain that is often associated with thioredoxin and glutaredoxin modules. | Isakov, N., et al. 2000. Trends Biochem Sci. 25: 537-9. PMID: 11084362
- Plasmodium falciparum possesses a classical glutaredoxin and a second, glutaredoxin-like protein with a PICOT homology domain. | Rahlfs, S., et al. 2001. J Biol Chem. 276: 37133-40. PMID: 11479312
- Tyrosine phosphorylation of PICOT and its translocation to the nucleus in response of human T cells to oxidative stress. | Babichev, Y. and Isakov, N. 2001. Adv Exp Med Biol. 495: 41-5. PMID: 11774602
- PICOT inhibits cardiac hypertrophy and enhances ventricular function and cardiomyocyte contractility. | Jeong, D., et al. 2006. Circ Res. 99: 307-14. PMID: 16809552
- Containing hypertrophy with a PICOT fence. | Dorn, GW. 2006. Circ Res. 99: 228-30. PMID: 16888246
- PICOT attenuates cardiac hypertrophy by disrupting calcineurin-NFAT signaling. | Jeong, D., et al. 2008. Circ Res. 102: 711-9. PMID: 18258855
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PICOT Antibody (D-10) | sc-390068 | 200 µg/ml | $316.00 | |||
PICOT Antibody (D-10): m-IgG Fc BP-HRP Bundle | sc-530056 | 200 µg Ab; 10 µg BP | $354.00 | |||
PICOT Antibody (D-10): m-IgGκ BP-HRP Bundle | sc-523394 | 200 µg Ab, 40 µg BP | $354.00 | |||
PICOT Antibody (D-10): m-IgG1 BP-HRP Bundle | sc-543844 | 200 µg Ab; 20 µg BP | $354.00 | |||
PICOT Antibody (D-10) AC | sc-390068 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PICOT Antibody (D-10) HRP | sc-390068 HRP | 200 µg/ml | $316.00 | |||
PICOT Antibody (D-10) FITC | sc-390068 FITC | 200 µg/ml | $330.00 | |||
PICOT Antibody (D-10) PE | sc-390068 PE | 200 µg/ml | $343.00 | |||
PICOT Antibody (D-10) Alexa Fluor® 488 | sc-390068 AF488 | 200 µg/ml | $357.00 | |||
PICOT Antibody (D-10) Alexa Fluor® 546 | sc-390068 AF546 | 200 µg/ml | $357.00 | |||
PICOT Antibody (D-10) Alexa Fluor® 594 | sc-390068 AF594 | 200 µg/ml | $357.00 | |||
PICOT Antibody (D-10) Alexa Fluor® 647 | sc-390068 AF647 | 200 µg/ml | $357.00 | |||
PICOT Antibody (D-10) Alexa Fluor® 680 | sc-390068 AF680 | 200 µg/ml | $357.00 | |||
PICOT Antibody (D-10) Alexa Fluor® 790 | sc-390068 AF790 | 200 µg/ml | $357.00 | |||
PICOT (D-10) Neutralizing Peptide | sc-390068 P | 100 µg/0.5 ml | $68.00 |