PICK1 Antibody (F-10): sc-166654

PICK1 Antibody (F-10) is a mouse monoclonal IgG1 antibody that detects PICK1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PICK1, or protein interacting with C kinase 1, is a PDZ-domain containing protein predominantly located in the perinuclear region of cells, where PICK1 plays a crucial role in synaptic transmission and cellular signaling. The localization of PICK1 allows for precise regulation of interactions with other proteins, such as the α-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) receptor, which is essential for excitatory synaptic transmission. By anchoring AMPA receptors at specific synapses, PICK1 contributes to the modulation of synaptic strength and plasticity, processes that are vital for learning and memory. Furthermore, PICK1 is phosphorylated in response to protein kinase C alpha (PKCα) activation, highlighting PICK1′s involvement in signaling pathways that regulate cell proliferation and differentiation. The unique PDZ-binding domain of PKCα, which is absent in other PKC isoforms, allows for specific interactions with PICK1, underscoring PICK1′s importance in cellular communication and function.

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PICK1 Antibody (F-10) References:

1. PICK1-ICA69 heteromeric BAR domain complex regulates synaptic targeting and surface expression of AMPA receptors.  |  Cao, M., et al. 2007. J Neurosci. 27: 12945-56. PMID: 18032668
2. PICK1 regulates incorporation of calcium-permeable AMPA receptors during cortical synaptic strengthening.  |  Clem, RL., et al. 2010. J Neurosci. 30: 6360-6. PMID: 20445062
3. PICK1 loss of function occludes homeostatic synaptic scaling.  |  Anggono, V., et al. 2011. J Neurosci. 31: 2188-96. PMID: 21307255
4. Membrane Binding and Modulation of the PDZ Domain of PICK1.  |  Erlendsson, S. and Madsen, KL. 2015. Membranes (Basel). 5: 597-615. PMID: 26501328
5. PICK1/calcineurin suppress ASIC1-mediated Ca2+ entry in rat pulmonary arterial smooth muscle cells.  |  Herbert, LM., et al. 2016. Am J Physiol Cell Physiol. 310: C390-400. PMID: 26702130
6. PICK1 is essential for insulin production and the maintenance of glucose homeostasis.  |  Li, J., et al. 2018. Mol Biol Cell. 29: 587-596. PMID: 29298842
7. PICK1-Deficient Mice Exhibit Impaired Response to Cocaine and Dysregulated Dopamine Homeostasis.  |  Jensen, KL., et al. 2018. eNeuro. 5: PMID: 29911172
8. Modulation of Hippocampal Network Oscillation by PICK1-Dependent Cell Surface Expression of mGlu3 Receptors.  |  Tuduri, P., et al. 2022. J Neurosci. 42: 8897-8911. PMID: 36202617
9. Sodium arsenite induces aggresome formation by promoting PICK1 BAR domain homodimer formation.  |  Lai, JHC., et al. 2024. Mol Biol Cell. 35: ar128. PMID: 39083353
10. PICK1 links KIBRA and AMPA receptor subunit GluA2 in coiled-coil-driven supramolecular complexes.  |  Shao, X. and Volk, L. 2025. J Biol Chem. 108397. PMID: 40074086

The preferred PICK1 antibody is PICK1 Antibody (D-10): sc-390479.