PIASy Antibody (H-9): sc-376315
- PIASy Antibody (H-9) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 61-130 mapping within an internal region of PIASy of mouse origin
- recommended for detection of PIASy of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PIASy Antibody (H-9) for WB applications. These reagents are now offered in bundles with PIASy Antibody (H-9) (see ordering information below).
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PIASy Antibody (H-9) is a mouse monoclonal IgG1 antibody that detects PIASy in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PIASy, a member of the protein inhibitor of activated signal transducer and activator of transcription (STAT) family, plays a crucial role in regulating cytokine signaling by acting as a negative regulator of STAT proteins, thereby modulating the intensity and duration of cellular responses to cytokines. This regulation is vital for maintaining cellular homeostasis and preventing excessive inflammatory responses, which can lead to various diseases. PIASy is primarily located in the nucleus, where PIASy functions as a transcriptional corepressor, particularly for the androgen receptor, and is involved in p53-mediated transcriptional regulation. PIASy′s ability to interact with these critical signaling pathways underscores PIASy′s importance in cellular processes such as proliferation, differentiation, and apoptosis. With versatile applications and significant role in cellular signaling, anti-PIASy antibody (H-9) serves as an essential tool for researchers studying cytokine signaling and its implications in health and disease.
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PIASy Antibody (H-9) References:
- Repression of Smad transcriptional activity by PIASy, an inhibitor of activated STAT. | Long, J., et al. 2003. Proc Natl Acad Sci U S A. 100: 9791-6. PMID: 12904571
- PIASy mediates NEMO sumoylation and NF-kappaB activation in response to genotoxic stress. | Mabb, AM., et al. 2006. Nat Cell Biol. 8: 986-93. PMID: 16906147
- PIASy controls ubiquitination-dependent proteasomal degradation of Ets-1. | Nishida, T., et al. 2007. Biochem J. 405: 481-8. PMID: 17456046
- PIASy represses CCAAT/enhancer-binding protein delta (C/EBPdelta) transcriptional activity by sequestering C/EBPdelta to the nuclear periphery. | Zhou, S., et al. 2008. J Biol Chem. 283: 20137-48. PMID: 18477566
- PIASy inhibits LRH-1-dependent CYP11A1 expression by competing for SRC-1 binding. | Hsieh, HT., et al. 2009. Biochem J. 419: 201-9. PMID: 19067654
- PIASy stimulates HIF1α SUMOylation and negatively regulates HIF1α activity in response to hypoxia. | Kang, X., et al. 2010. Oncogene. 29: 5568-78. PMID: 20661221
- Protein Inhibitor of Activated STAT Y (PIASy) Regulates Insulin Secretion by Interacting with LIM Homeodomain Transcription Factor Isl1. | Yan, C., et al. 2016. Sci Rep. 6: 39308. PMID: 28000708
- Identification of a new small ubiquitin-like modifier (SUMO)-interacting motif in the E3 ligase PIASy. | Kaur, K., et al. 2017. J Biol Chem. 292: 10230-10238. PMID: 28455449
- SUMO E3 Ligase PIASy Mediates High Glucose-Induced Activation of NF-κB Inflammatory Signaling in Rat Mesangial Cells. | Huang, W., et al. 2017. Mediators Inflamm. 2017: 1685194. PMID: 29038616
- Alcohol-induced autophagy via upregulation of PIASy promotes HCV replication in human hepatoma cells. | Ran, M., et al. 2018. Cell Death Dis. 9: 898. PMID: 30185779
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PIASy Antibody (H-9) | sc-376315 | 200 µg/ml | $316.00 | |||
PIASy Antibody (H-9): m-IgG Fc BP-HRP Bundle | sc-540545 | 200 µg Ab; 10 µg BP | $354.00 | |||
PIASy Antibody (H-9): m-IgG1 BP-HRP Bundle | sc-542196 | 200 µg Ab; 20 µg BP | $354.00 |