PI 3-kinase p110 Antibody (D-4): sc-8010

PI 3-kinase p110 Antibody (D-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects PI 3-kinase p110 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PI 3-kinase p110 (D-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The phosphatidylinositol 3-kinase (PI 3-kinase) pathway plays a crucial role in cellular functions such as growth, proliferation, and survival, making the study of PI 3-kinase components essential for understanding various diseases, including cancer. PI 3-kinase p110 subunit is responsible for the catalytic activity of the enzyme, converting phosphatidylinositol-4,5-bisphosphate into phosphatidylinositol-3,4,5-trisphosphate, a key signaling molecule that activates downstream pathways. The regulation of this pathway is vital, as dysregulation can lead to uncontrolled cell growth and tumorigenesis. Additionally, p110 isoforms exhibit distinct tissue distributions and regulatory mechanisms, with p110α and p110β being widely expressed, while p110δ is primarily found in white blood cells, highlighting the importance of understanding the specific roles of each isoform in various biological contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PI 3-kinase p110 Antibody (D-4) References:

1. Phosphatidylinositol 3-kinase: structure and expression of the 110 kd catalytic subunit.  |  Hiles, ID., et al. 1992. Cell. 70: 419-29. PMID: 1322797
2. ANG II enhances contractile responses via PI3-kinase p110 delta pathway in aortas from diabetic rats with systemic hyperinsulinemia.  |  Kobayashi, T., et al. 2006. Am J Physiol Heart Circ Physiol. 291: H846-53. PMID: 16517941
3. Characterization of two 85 kd proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase.  |  Otsu, M., et al. 1991. Cell. 65: 91-104. PMID: 1707345
4. Cloning of PI3 kinase-associated p85 utilizing a novel method for expression/cloning of target proteins for receptor tyrosine kinases.  |  Skolnik, EY., et al. 1991. Cell. 65: 83-90. PMID: 1849461
5. The P110 subunit of PI3-K is a therapeutic target of acacetin in skin cancer.  |  Jung, SK., et al. 2014. Carcinogenesis. 35: 123-30. PMID: 23913940
6. Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase.  |  Stoyanov, B., et al. 1995. Science. 269: 690-3. PMID: 7624799
7. Cloning of a novel, ubiquitously expressed human phosphatidylinositol 3-kinase and identification of its binding site on p85.  |  Hu, P., et al. 1993. Mol Cell Biol. 13: 7677-88. PMID: 8246984
8. Dominant negative effect of the truncated p110 subunit of phosphatidylinositol-3 kinase.  |  Takayanagi, J., et al. 1996. Biochem Mol Biol Int. 39: 721-8. PMID: 8843340
9. P110delta, a novel phosphoinositide 3-kinase in leukocytes.  |  Vanhaesebroeck, B., et al. 1997. Proc Natl Acad Sci U S A. 94: 4330-5. PMID: 9113989
10. Gbetagamma stimulates phosphoinositide 3-kinase-gamma by direct interaction with two domains of the catalytic p110 subunit.  |  Leopoldt, D., et al. 1998. J Biol Chem. 273: 7024-9. PMID: 9507010
11. Downregulated expression of the signaling molecules Nck, c-Crk, Grb2/Ash, PI 3-kinase p110 alpha and WRN during fibroblast aging in vitro.  |  Matuoka, K. and Takenawa, T. 1998. Biochim Biophys Acta. 1401: 211-5. PMID: 9531977
12. Binding of CD4 ligands induces tyrosine phosphorylation of phosphatidylinositol-3 kinase p110 subunit.  |  Mazerolles, F. and Fischer, A. 1998. Int Immunol. 10: 1897-905. PMID: 9885911

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PI 3-kinase p110 Antibody (D-4) has 43 citations in a variety of scientific publications.

Primary antibodies like Anti-PI 3-kinase p110 Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PI 3-kinase p110 Antibody (D-4) Specifications

Alternate Product Name: PI3K p110

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 800-1039

Epitope Target: PI 3-kinase p110

Epitope Species: human

Product Reactivity/Detection: PI 3-kinase p110

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 43 citations in various scientific publications

Available Conjugates/Forms for PI 3-kinase p110 Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin