PI 3-kinase p100 Antibody (F-11): sc-365404

PI 3-kinase p100 Antibody (F-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects PI 3-kinase p100 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PI 3-kinase p100 (F-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PI 3-kinase p100, also known as hVps34 or PIK3C3, plays a crucial role in cellular processes such as endosome to lysosome transport, autophagy, and the formation of multivesicular bodies, which are essential for the degradation and recycling of cellular components. PI 3-kinase p100 is primarily located in the cytoplasm and is ubiquitously expressed, with significant levels found in skeletal muscle. PI 3-kinase p100 localization is vital for function in membrane trafficking and nutrient sensing, as PI 3-kinase p100 is involved in the phosphorylation of phosphatidylinositol to generate PtdIns3P, a lipid that serves as a signaling molecule in various cellular pathways. Notably, PI 3-kinase p100 operates differently from class I PI3Ks, as PI 3-kinase p100 activity is enhanced by manganese rather than magnesium, and PI 3-kinase p100 is subject to regulation by nutrient availability, highlighting PI 3-kinase p100′s importance in the mTOR signaling pathway and autophagy regulation.

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PI 3-kinase p100 Antibody (F-11) References:

1. Identification of PIK3C3 promoter variant associated with bipolar disorder and schizophrenia.  |  Stopkova, P., et al. 2004. Biol Psychiatry. 55: 981-8. PMID: 15121481
2. TbVps34, the trypanosome orthologue of Vps34, is required for Golgi complex segregation.  |  Hall, BS., et al. 2006. J Biol Chem. 281: 27600-12. PMID: 16835237
3. The evolutionarily conserved domain of Beclin 1 is required for Vps34 binding, autophagy and tumor suppressor function.  |  Furuya, N., et al. 2005. Autophagy. 1: 46-52. PMID: 16874027
4. Regulation of class III (Vps34) PI3Ks.  |  Yan, Y. and Backer, JM. 2007. Biochem Soc Trans. 35: 239-41. PMID: 17371248
5. Beclin 1-independent pathway of damage-induced mitophagy and autophagic stress: implications for neurodegeneration and cell death.  |  Chu, CT., et al. 2007. Autophagy. 3: 663-6. PMID: 17622797
6. mVps34 is activated by an acute bout of resistance exercise.  |  Mackenzie, MG., et al. 2007. Biochem Soc Trans. 35: 1314-6. PMID: 17956340
7. Resveratrol-induced apoptosis depends on the lipid kinase activity of Vps34 and on the formation of autophagolysosomes.  |  Trincheri, NF., et al. 2008. Carcinogenesis. 29: 381-9. PMID: 18048384
8. The regulation and function of Class III PI3Ks: novel roles for Vps34.  |  Backer, JM. 2008. Biochem J. 410: 1-17. PMID: 18215151
9. Autophagy-related protein PIK3C3/VPS34 controls T cell metabolism and function.  |  Yang, G., et al. 2021. Autophagy. 17: 1193-1204. PMID: 32268825
10. SRSF1 inhibits autophagy through regulating Bcl-x splicing and interacting with PIK3C3 in lung cancer.  |  Lv, Y., et al. 2021. Signal Transduct Target Ther. 6: 108. PMID: 33664238
11. Pik3c3 expression profiling in the mouse kidney and its role in proximal tubule cell physiology.  |  Liu, T., et al. 2024. Am J Physiol Cell Physiol. 327: C1094-C1110. PMID: 39250817
12. A human phosphatidylinositol 3-kinase complex related to the yeast Vps34p-Vps15p protein sorting system.  |  Volinia, S., et al. 1995. EMBO J. 14: 3339-48. PMID: 7628435

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PI 3-kinase p100 Antibody (F-11) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-PI 3-kinase p100 Antibody (F-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PI 3-kinase p100 Antibody (F-11) Specifications

Alternate Product Name: PI3K p100

Product Clone Name: F-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: PI 3-kinase p100

Epitope Species: human

Product Reactivity/Detection: PI 3-kinase p100

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for PI 3-kinase p100 Antibody (F-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin