Anti-PI 3-kinase C2 beta Antibody (A-3) recommended for detection of PI 3-kinase C2β of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-PI 3-kinase C2β Antibody (A-3): sc-377064

Anti-PI 3-kinase C2β Antibody (A-3) is rated 4.7 out of 5 by 6.
  • y_2022, m_5, d_24, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.27
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_en_US, sid_377064, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 124ms
  • REVIEWS, PRODUCT
loading
5
4
4
2
3
0
2
0
1
0

Datasheets

    • Anti-PI 3-kinase C2β Antibody (A-3) is a mouse monoclonal IgG1 κ PI 3-kinase C2β antibody, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of PI 3-kinase C2β of human origin
    • Anti-PI 3-kinase C2 beta Antibody (A-3) is recommended for detection of PI 3-kinase C2β of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-PI 3-kinase C2 beta Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PI 3-kinase C2β (A-3): sc-377064.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PI 3-kinase C2β Antibody (A-3) for WB applications. These reagents are now offered in bundles with PI 3-kinase C2β Antibody (A-3) (see ordering information below).
loading

What is the recommended fixation method for immunofluorescence staining with PI 3-kinase C2β (A-3): sc-377064 monoclonal antibody?

Asked by: jenniferc
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
  • y_2022, m_5, d_24, h_21CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.27
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_377064, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 103ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Sal This antibody works fine with western blot I she tried with Jurkat T cells
Date published: 2017-10-17
Rated 4 out of 5 by from Not quite clear. 1:200 diluted for wb of rat protein,the band is a little weak.
Date published: 2017-08-23
Rated 5 out of 5 by from Great IF and WB results Very good reagent for both IHC staining and western blot applications
Date published: 2017-05-31
Rated 4 out of 5 by from This antibody worked good. Tested in human thyroid cell lines, specific band obtained with good signal.
Date published: 2017-05-25
Rated 5 out of 5 by from Good signal strength in human bladder and Good signal strength in human bladder and human heart tissue extracts. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from Works well for IF applications Works well for IF applications, cytoplasmic staining in MeOH fixed HeLa cells. -SCBT QC
Date published: 2015-01-21
  • y_2022, m_5, d_24, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.27
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_en_US, sid_377064, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 17ms
  • REVIEWS, PRODUCT