PGK1 Antibody (14): sc-130335

PGK1 Antibody (14) is a mouse monoclonal IgG1 kappa light chain antibody that detects phosphoglycerate kinase 1 (PGK1) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-PGK1 antibody (14) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PGK1 plays a crucial role in cellular metabolism as a key glycolytic enzyme, catalyzing the conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate while generating adenosine triphosphate (ATP) from adenosine diphosphate (ADP). This enzymatic activity is vital for energy production in cells, particularly under anaerobic conditions, and is essential for sustaining cellular functions and proliferation. The human PGK gene, located on the X chromosome at position Xq21.1, is associated with various genetic disorders, including prostate cancer and androgen insensitivity, highlighting PGK1′s importance in both normal physiology and disease states. Additionally, PGK1 is secreted by tumor cells, where anti-PGK1 antibody (14) acts as a disulfide reductase, facilitating angiogenesis by reducing disulfide bonds in proteins such as plasmin, which is critical for the release of angiostatin, a potent inhibitor of tumor blood vessel formation. This multifaceted role of PGK1 underscores PGK1′s significance not only in metabolic pathways but also in cancer biology, making anti-PGK1 antibody (14) a valuable tool for research in these areas.

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PGK1 Antibody (14) References:

1. A hemizygous short tandem repeat polymorphism 3' to the human phosphoglycerate kinase gene.  |  Riley, DE., et al. 1999. Mol Biol Rep. 26: 159-65. PMID: 10532310
2. Involvement of a cellular glycolytic enzyme, phosphoglycerate kinase, in Sendai virus transcription.  |  Ogino, T., et al. 1999. J Biol Chem. 274: 35999-6008. PMID: 10585490
3. Phosphoglycerate kinase acts in tumour angiogenesis as a disulphide reductase.  |  Lay, AJ., et al. 2000. Nature. 408: 869-73. PMID: 11130727
4. Structure of the human phosphoglycerate kinase gene and the intron-mediated evolution and dispersal of the nucleotide-binding domain.  |  Michelson, AM., et al. 1985. Proc Natl Acad Sci U S A. 82: 6965-9. PMID: 2995995
5. Macrophage-Associated PGK1 Phosphorylation Promotes Aerobic Glycolysis and Tumorigenesis.  |  Zhang, Y., et al. 2018. Mol Cell. 71: 201-215.e7. PMID: 30029001
6. PTEN Suppresses Glycolysis by Dephosphorylating and Inhibiting Autophosphorylated PGK1.  |  Qian, X., et al. 2019. Mol Cell. 76: 516-527.e7. PMID: 31492635
7. Localisation of PGK1 determines metabolic phenotype to balance metastasis and proliferation in patients with SMAD4-negative pancreatic cancer.  |  Liang, C., et al. 2020. Gut. 69: 888-900. PMID: 31611300
8. O-GlcNAcylation of PGK1 coordinates glycolysis and TCA cycle to promote tumor growth.  |  Nie, H., et al. 2020. Nat Commun. 11: 36. PMID: 31911580
9. FOXO3A-induced LINC00926 suppresses breast tumor growth and metastasis through inhibition of PGK1-mediated Warburg effect.  |  Chu, Z., et al. 2021. Mol Ther. 29: 2737-2753. PMID: 33940159
10. LncRNA NEAT1 facilitates glioma progression via stabilizing PGK1.  |  Liang, J., et al. 2022. J Transl Med. 20: 80. PMID: 35123484
11. HIF1α/ATF3 partake in PGK1 K191/K192 succinylation by modulating P4HA1/succinate signaling in glioblastoma.  |  Yang, S., et al. 2024. Neuro Oncol. 26: 1405-1420. PMID: 38441561
12. Hypoxia-induced downregulation of PGK1 crotonylation promotes tumorigenesis by coordinating glycolysis and the TCA cycle.  |  Guo, Z., et al. 2024. Nat Commun. 15: 6915. PMID: 39134530

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PGK1 Antibody (14) has 37 citations in a variety of scientific publications.

Primary antibodies like Anti-PGK1 Antibody (14) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PGK1 Antibody (14) Specifications

Alternate Product Name: Phosphoglycerate Kinase

Product Clone Name: 14

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: PGK1

Epitope Species: human

Product Reactivity/Detection: PGK1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 37 citations in various scientific publications

Available Conjugates/Forms for PGK1 Antibody (14): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred PGK1/2 antibody is PGK1/2 Antibody (A-5): sc-48342.