PGD Antibody (G-2): sc-398977

PGD Antibody (G-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects PGD protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PGD (G-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PGD protein, also known as phosphogluconate dehydrogenase or 6PGD, is a crucial enzyme consisting of 483 amino acids that plays a vital role in the pentose phosphate pathway, which is essential for nucleic acid biosynthesis and serves as a significant source of NADPH. This pathway is particularly important for cellular processes such as fatty acid synthesis and maintenance of redox balance. PGD catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate, releasing carbon dioxide and reducing NADP in the process. Notably, PGD deficiency can lead to increased erythrocyte pyruvate kinase activity and decreased glutathione synthetase levels, resulting in hemolysis, although defects in PGD are often asymptomatic and inherited in an autosomal dominant manner. The catalytic regions of PGD, including the substrate and coenzyme binding sites, are highly conserved across various species, underscoring PGD′s evolutionary significance and functional importance in metabolic pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PGD Antibody (G-2) References:

1. 6-Phosphogluconate dehydrogenase deficiency in an Italian family.  |  Caprari, P., et al. 2001. Ann Hematol. 80: 41-4. PMID: 11233775
2. [PGD(2)/L-PGDS system in hypertension and renal injury].  |  Uehara, Y. 2004. Nihon Yakurigaku Zasshi. 123: 23-33. PMID: 14695455
3. 6-phosphogluconate dehydrogenase: a target for drugs in African trypanosomes.  |  Hanau, S., et al. 2004. Curr Med Chem. 11: 2639-50. PMID: 15544466
4. Functional constraints of 6-phosphogluconate dehydrogenase (6-PGD) based on sequence and structural information.  |  Goulielmos, GN., et al. 2004. J Mol Evol. 59: 358-71. PMID: 15553090
5. Purification and kinetic properties of 6-phosphogluconate dehydrogenase from rat small intestine.  |  Ceyhan, D., et al. 2005. Protein J. 24: 293-301. PMID: 16284727
6. PGD for cystic fibrosis patients and couples at risk of an additional genetic disorder combined with 24-chromosome aneuploidy testing.  |  Rechitsky, S., et al. 2013. Reprod Biomed Online. 26: 420-30. PMID: 23523379
7. Implementing PGD/PGD-A in IVF clinics: considerations for the best laboratory approach and management.  |  Capalbo, A., et al. 2016. J Assist Reprod Genet. 33: 1279-1286. PMID: 27423665
8. Primary Graft Dysfunction (PGD) Following Lung Transplantation.  |  Shah, RJ. and Diamond, JM. 2018. Semin Respir Crit Care Med. 39: 148-154. PMID: 29590671
9. Review: Preimplantation genetic diagnosis (PGD) as a reproductive option in patients with neurodegenerative disorders.  |  Sciorio, R., et al. 2021. Reprod Biol. 21: 100468. PMID: 33321391
10. Genetic linkage relations of the loci for 6-phosphogluconate dehydrogenase and adenosine deaminase in man.  |  Weitkamp, LR., et al. 1970. Am J Hum Genet. 22: 216-20. PMID: 4985154
11. Slow-binding inhibition of 6-phosphogluconate dehydrogenase by zinc ion.  |  Niehaus, WG., et al. 1996. Arch Biochem Biophys. 333: 333-7. PMID: 8809071
12. 6-Phosphogluconate dehydrogenase: the mechanism of action investigated by a comparison of the enzyme from different species.  |  Rippa, M., et al. 1998. Biochim Biophys Acta. 1429: 83-92. PMID: 9920387

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PGD Antibody (G-2) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-PGD Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PGD Antibody (G-2) Specifications

Alternate Product Name: PGD

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 417-443

Epitope Target: PGD

Epitope Species: human

Product Reactivity/Detection: PGD

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for PGD Antibody (G-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin