PFK-1 Antibody (G-11): sc-166722

PFK-1 Antibody (G-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects PFK-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PFK-1 (G-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PFK-1, also known as phosphofructokinase-1, plays a crucial role in glycolysis by catalyzing the conversion of fructose 6-phosphate and ATP into fructose 1,6-bisphosphate, a key regulatory step in glucose metabolism. This enzymatic activity maintains energy homeostasis in cells, particularly during periods of high energy demand. PFK-1 functions as a tetrameric enzyme composed of different isoforms, including muscle (PFKM), liver (PFKL), and platelet (PFKP) types, each regulated by various metabolites. ATP and citrate inhibit PFK-1, signaling sufficient energy levels, while elevated AMP and fructose-2,6-bisphosphate activate PFK-1, with fructose-2,6-bisphosphate being produced by the bifunctional enzyme PFK-2. This intricate metabolite regulation of PFK-1 underscores its importance in metabolic pathways and highlights potential therapeutic interventions in metabolic disorders.

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PFK-1 Antibody (G-11) References:

1. Role of Ser530, Arg292, and His662 in the allosteric behavior of rabbit muscle phosphofructokinase.  |  Chang, SH. and Kemp, RG. 2002. Biochem Biophys Res Commun. 290: 670-5. PMID: 11785951
2. Changes in activity and expression of phosphofructokinase in different rat brain regions after basal forebrain cholinergic lesion.  |  Zeitschel, U., et al. 2002. J Neurochem. 83: 371-80. PMID: 12423247
3. The a-subunit of the V-type H+-ATPase interacts with phosphofructokinase-1 in humans.  |  Su, Y., et al. 2003. J Biol Chem. 278: 20013-8. PMID: 12649290
4. Phosphofructokinase muscle-specific isoform requires caveolin-3 expression for plasma membrane recruitment and caveolar targeting: implications for the pathogenesis of caveolin-related muscle diseases.  |  Sotgia, F., et al. 2003. Am J Pathol. 163: 2619-34. PMID: 14633633
5. No spontaneous second wind in muscle phosphofructokinase deficiency.  |  Haller, RG. and Vissing, J. 2004. Neurology. 62: 82-6. PMID: 14718702
6. Novel testis- and embryo-specific isoforms of the phosphofructokinase-1 muscle type gene.  |  Yamada, S., et al. 2004. Biochem Biophys Res Commun. 316: 580-7. PMID: 15020257
7. Interaction of calmodulin with the phosphofructokinase target sequence.  |  Martin, SR., et al. 2004. FEBS Lett. 577: 284-8. PMID: 15527800
8. Expression of caveolin-1 in lymphocytes induces caveolae formation and recruitment of phosphofructokinase to the plasma membrane.  |  Vallejo, J. and Hardin, CD. 2005. FASEB J. 19: 586-7. PMID: 15665033
9. Down regulation of tumour biomarkers in colon cancer cells with IRNA PFK-1 plus metformin.  |  Reyes Serratos, EA., et al. 2021. J BUON. 26: 1210-1218. PMID: 34564972
10. MFN2 Prevents Neointimal Hyperplasia in Vein Grafts via Destabilizing PFK1.  |  Tang, Y., et al. 2022. Circ Res. 130: e26-e43. PMID: 35450439
11. The mechanism of PFK-1 in the occurrence and development of bladder cancer by regulating ZEB1 lactylation.  |  Wang, R., et al. 2024. BMC Urol. 24: 59. PMID: 38481182
12. Endogenous nitric oxide inhibits glucose-induced insulin secretion by suppression of phosphofructokinase activity in pancreatic islets.  |  Tsuura, Y., et al. 1998. Biochem Biophys Res Commun. 252: 34-8. PMID: 9813142

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PFK-1 Antibody (G-11) has 31 citations in a variety of scientific publications.

Primary antibodies like Anti-PFK-1 Antibody (G-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PFK-1 Antibody (G-11) Specifications

Alternate Product Name: PFKM

Product Clone Name: G-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 676-730

Epitope Target: PFK-1

Epitope Species: human

Product Reactivity/Detection: muscle type PFK-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 31 citations in various scientific publications

Available Conjugates/Forms for PFK-1 Antibody (G-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred PFK-1 antibody is PFK-1 Antibody (E-4): sc-377346.