PEPD Antibody (A-3): sc-390042

PEPD Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects PEPD protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PEPD Antibody (A-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PEPD, also known as prolidase, is a cytosolic dipeptidase that belongs to the peptidase M24B family and plays a crucial role in collagen metabolism and proline recycling, which is vital for protein synthesis and cellular function. This enzyme hydrolyzes di- and tripeptides containing proline or hydroxyproline at the C-terminus, functioning as a homodimer. Deficiencies in PEPD lead to prolidase deficiency, an autosomal recessive disorder characterized by iminodipeptiduria, skin ulcers, mental retardation, recurrent infections, and distinctive facial features. Mutations in PEPD have been implicated in systemic lupus erythematosus and necrosis-like cell death in fibroblasts, highlighting PEPD′s significance in various pathological conditions. Research suggests a strong association between prolidase polymorphisms and myotonic dystrophy trait, underscoring PEPD′s importance in both health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PEPD Antibody (A-3) References:

1. A novel nonsense mutation of the PEPD gene in a Japanese patient with prolidase deficiency.  |  Kikuchi, S., et al. 2000. J Hum Genet. 45: 102-4. PMID: 10721675
2. Mutation analysis of five new patients affected by prolidase deficiency: the lack of enzyme activity causes necrosis-like cell death in cultured fibroblasts.  |  Forlino, A., et al. 2002. Hum Genet. 111: 314-22. PMID: 12384772
3. The promoter region of the Escherichia coli pepD gene: deletion analysis and control by phosphate concentration.  |  Henrich, B., et al. 1992. Mol Gen Genet. 232: 117-25. PMID: 1313142
4. A nonsense mutation of PEPD in four Amish children with prolidase deficiency.  |  Wang, H., et al. 2006. Am J Med Genet A. 140: 580-5. PMID: 16470701
5. Molecular basis of prolidase (peptidase D) deficiency.  |  Endo, F. and Matsuda, I. 1991. Mol Biol Med. 8: 117-27. PMID: 1943683
6. Structural organization of the gene for human prolidase (peptidase D) and demonstration of a partial gene deletion in a patient with prolidase deficiency.  |  Tanoue, A., et al. 1990. J Biol Chem. 265: 11306-11. PMID: 1972707
7. Prolidase deficiency: biochemical classification of alleles.  |  Boright, AP., et al. 1989. Am J Hum Genet. 44: 731-40. PMID: 2705457
8. Prolidase deficiency in two siblings with chronic leg ulcerations. Clinical, biochemical, and morphologic aspects.  |  Leoni, A., et al. 1987. Arch Dermatol. 123: 493-9. PMID: 3827281
9. Four novel PEPD alleles causing prolidase deficiency.  |  Ledoux, P., et al. 1994. Am J Hum Genet. 54: 1014-21. PMID: 8198124

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PEPD Antibody (A-3) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-PEPD Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PEPD Antibody (A-3) Specifications

Alternate Product Name: PEPD

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 101-305

Epitope Target: PEPD

Epitope Species: human

Product Reactivity/Detection: PEPD

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for PEPD Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin