PEPCK-C/PCK1 Antibody (F-11): sc-377027

PEPCK-C Antibody (F-11) is a mouse monoclonal IgG2a kappa light chain antibody designed to target Phosphoenolpyruvate carboxykinase-C (PEPCK-C), also known as PCK1. PEPCK-C monoclonal antibody (F-11) is raised against an epitope spanning amino acids 189-221 within an internal region of human PEPCK-C, ensuring precise recognition and detection in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PEPCK-C is a crucial enzyme in the gluconeogenesis pathway, responsible for the synthesis of glucose from non-carbohydrate sources in liver cells, thereby maintaining blood glucose levels during fasting and metabolic stress. Structurally, PEPCK-C interacts with several key proteins, including the transcription factor FOXO1 and the coactivator PGC-1α, which regulate its expression in response to hormonal signals like insulin and glucagon. These interactions are essential for the dynamic control of glucose production and metabolic homeostasis. Dysregulation of PEPCK-C and its interacting partners is closely associated with metabolic disorders such as type 2 diabetes and obesity, highlighting its significance as a therapeutic target. PEPCK-C (F-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, providing flexibility for diverse experimental needs. By enabling the precise detection and study of PEPCK-C and its interactions with other proteins, PEPCK-C (F-11) monoclonal antibody supports advanced research into the molecular mechanisms of glucose metabolism and the development of potential treatments for insulin resistance and related metabolic conditions.

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PEPCK-C/PCK1 Antibody (F-11) References:

1. [Novel aspects in the mechanisms of steroid diabetes and the regulation of hepatic glucose production by insulin and steroids].  |  Barthel, A., et al. 2003. Med Klin (Munich). 98: 283-6. PMID: 12721674
2. Identification of a novel variant in the phosphoenolpyruvate carboxykinase gene promoter in Japanese patients with type 2 diabetes.  |  Horikawa, Y., et al. 2003. Horm Metab Res. 35: 308-12. PMID: 12916001
3. Novel concepts in insulin regulation of hepatic gluconeogenesis.  |  Barthel, A. and Schmoll, D. 2003. Am J Physiol Endocrinol Metab. 285: E685-92. PMID: 12959935
4. Sustained peripheral expression of transgene adiponectin offsets the development of diet-induced obesity in rats.  |  Shklyaev, S., et al. 2003. Proc Natl Acad Sci U S A. 100: 14217-22. PMID: 14617771
5. AMP-activated protein kinase regulates PEPCK gene expression by direct phosphorylation of a novel zinc finger transcription factor.  |  Inoue, E. and Yamauchi, J. 2006. Biochem Biophys Res Commun. 351: 793-9. PMID: 17097062
6. Insulin and other regulatory factors modulate the growth and the phosphoenolpyruvate carboxykinase (PEPCK) activity of primary rabbit kidney proximal tubule cells in serum free medium.  |  Wang, Y. and Taub, M. 1991. J Cell Physiol. 147: 374-82. PMID: 1710231
7. Structures of rat cytosolic PEPCK: insight into the mechanism of phosphorylation and decarboxylation of oxaloacetic acid.  |  Sullivan, SM. and Holyoak, T. 2007. Biochemistry. 46: 10078-88. PMID: 17685635
8. Identification of a sequence in the PEPCK gene that mediates a negative effect of insulin on transcription.  |  O'Brien, RM., et al. 1990. Science. 249: 533-7. PMID: 2166335
9. Insulin decreases H4IIE cell PEPCK mRNA by a mechanism that does not involve cAMP.  |  Beale, EG., et al. 1986. Diabetes. 35: 546-9. PMID: 3007246

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PEPCK-C/PCK1 Antibody (F-11) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-PEPCK-C/PCK1 Antibody (F-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PEPCK-C/PCK1 Antibody (F-11) Specifications

Alternate Product Name: PEPCK-C

Product Clone Name: F-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 189-221

Epitope Target: PEPCK-C

Epitope Species: human

Product Reactivity/Detection: PEPCK-C

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for PEPCK-C/PCK1 Antibody (F-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred PEPCK/PCK1/PCK2 antibody is PEPCK/PCK1/PCK2 Antibody (F-3): sc-271029.