PEPCK/PCK1/PCK2 Antibody (F-2): sc-166778

PEPCK Antibody (F-2) is a mouse monoclonal IgG1 kappa light chain antibody designed to target Phosphoenolpyruvate carboxykinase (PEPCK), also known as PCK1 and PCK2. Anti-PEPCK antibody (F-2) recognizes a precise epitope spanning amino acids 428-461 near the C-terminus of human PEPCK, ensuring accurate detection of both cytosolic form (PEPCK-C) and mitochondrial form (PEPCK-M) across mouse, rat, human, equine, canine, bovine, porcine, and avian species. PEPCK is a critical enzyme in gluconeogenesis, responsible for glucose synthesis in liver, which is essential for maintaining blood glucose levels, especially during fasting. The regulation of PEPCK by hormones such as glucagon and glucocorticoids, which upregulate expression, and insulin, which suppresses expression, highlights a pivotal role in metabolic homeostasis. Dysregulation of PEPCK activity is closely associated with type 2 diabetes development, making PEPCK an important target for therapeutic interventions aimed at treating insulin resistance and obesity. PEPCK monoclonal antibody (F-2) is versatile in applications, suitable for western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Additionally, PEPCK (F-2) antibody is available in various conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, as well as an agarose-conjugated form, providing flexibility for diverse experimental requirements. By facilitating precise detection and study of PEPCK, anti-PEPCK antibody (F-2) supports advanced research into metabolic diseases and potential therapeutic strategies.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PEPCK/PCK1/PCK2 Antibody (F-2) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-PEPCK/PCK1/PCK2 Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PEPCK/PCK1/PCK2 Antibody (F-2) Specifications

Alternate Product Name: PEPCK

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 428-461

Epitope Target: Phosphoenolpyruvate carboxykinase

Epitope Species: human

Product Reactivity/Detection: PEPCK-M and PEPCK-C

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for PEPCK/PCK1/PCK2 Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred PEPCK/PCK1/PCK2 antibody is PEPCK/PCK1/PCK2 Antibody (F-3): sc-271029.