PEPCK Antibody (F-2)recommended for detection of PEPCK-M and PEPCK-C of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA

PEPCK Antibody (F-2): sc-166778

PEPCK Antibody (F-2) is rated 5.0 out of 5 by 4.
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Datasheets

    • PEPCK Antibody (F-2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 428-461 near the C-terminus of Phosphoenolpyruvate carboxykinase of human origin
    • PEPCK Antibody (F-2) is recommended for detection of PEPCK-M and PEPCK-C of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • PEPCK Antibody (F-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-166778 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PEPCK (F-2): sc-166778.
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What application is the blocking peptide sc-166778 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Best choice for PEPCK detection Beautiful distinct and clean bands for WB in KNRK and Hep G2 whole cell lysates. Used at a 1:200 dilution.
Date published: 2017-01-19
Rated 5 out of 5 by from Good specificity in WB in human liver tissue Good specificity in WB in human liver tissue extract. -SCBT QC
Date published: 2015-05-18
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic staining Great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-02-05
Rated 5 out of 5 by from Strong band in WB in KNRK whole cell lysate Strong band in WB in KNRK whole cell lysate. -SCBT QC
Date published: 2015-02-04
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