PDPc Antibody (D-11): sc-398117
- PDPc Antibody (D-11) is a mouse monoclonal IgG1 κ PDPc antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-156 mapping at the N-terminus of PDPc of human origin
- PDPc Antibody (D-11) is recommended for detection of PDPc of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PDPc Antibody (D-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PDPc Antibody (D-11) for WB applications. These reagents are now offered in bundles with PDPc Antibody (D-11) (see ordering information below).
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PDPc Antibody (D-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects PDPc protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PDPc (D-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The pyruvate dehydrogenase phosphatase (PDP) is a crucial serine phosphatase that plays a vital role in cellular metabolism by catalyzing the dephosphorylation and reactivation of the alpha subunit of the E1 component of the mitochondrial pyruvate dehydrogenase multienzyme complex. PDP is composed of a heterodimer of catalytic and regulatory subunits, with PDPc (pyruvate dehydrogenase phosphatase, catalytic subunit 1), a 537 amino acid protein localized within the mitochondrial matrix, being particularly significant. PDPc efficiently dephosphorylates all three phosphorylation sites on the alpha chain of the E1 component, thereby activating pyruvate dehydrogenase to facilitate the conversion of pyruvate to acetyl-CoA, a critical substrate for the Krebs cycle. Additionally, PDPc is stimulated by calcium binding and requires two magnesium ions as cofactors for enzymatic activity. Defects in the gene encoding PDPc can lead to pyruvate dehydrogenase phosphatase deficiency, resulting in serious metabolic disorders such as lactic acidosis and neurological dysfunction, underscoring PDPc′s essential role in maintaining metabolic homeostasis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PDPc Antibody (D-11) References:
- Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning. | Hu, RM., et al. 2000. Proc Natl Acad Sci U S A. 97: 9543-8. PMID: 10931946
- Regulation of mammalian pyruvate dehydrogenase complex by phosphorylation: complexity of multiple phosphorylation sites and kinases. | Patel, MS. and Korotchkina, LG. 2001. Exp Mol Med. 33: 191-7. PMID: 11795479
- Characterization of the isozymes of pyruvate dehydrogenase phosphatase: implications for the regulation of pyruvate dehydrogenase activity. | Karpova, T., et al. 2003. Biochim Biophys Acta. 1652: 126-35. PMID: 14644048
- Probing a putative active site of the catalytic subunit of pyruvate dehydrogenase phosphatase 1 (PDP1c) by site-directed mutagenesis. | Karpova, T., et al. 2004. Biochim Biophys Acta. 1700: 43-51. PMID: 15210124
- Pyruvate dehydrogenase phosphatase deficiency: identification of the first mutation in two brothers and restoration of activity by protein complementation. | Maj, MC., et al. 2005. J Clin Endocrinol Metab. 90: 4101-7. PMID: 15855260
- Crystal structure of pyruvate dehydrogenase phosphatase 1 and its functional implications. | Vassylyev, DG. and Symersky, J. 2007. J Mol Biol. 370: 417-26. PMID: 17532339
- Chronic treatment with aripiprazole induces differential gene expression in the rat frontal cortex. | Cheng, MC., et al. 2008. Int J Neuropsychopharmacol. 11: 207-16. PMID: 17868501
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDPc Antibody (D-11) | sc-398117 | 200 µg/ml | $322.00 | |||
PDPc Antibody (D-11): m-IgG Fc BP-HRP Bundle | sc-530697 | 200 µg Ab; 10 µg BP | $361.00 | |||
PDPc Antibody (D-11): m-IgGκ BP-HRP Bundle | sc-524223 | 200 µg Ab, 40 µg BP | $361.00 | |||
PDPc Antibody (D-11): m-IgG1 BP-HRP Bundle | sc-544205 | 200 µg Ab; 20 µg BP | $361.00 | |||
PDPc Antibody (D-11) AC | sc-398117 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PDPc Antibody (D-11) HRP | sc-398117 HRP | 200 µg/ml | $322.00 | |||
PDPc Antibody (D-11) FITC | sc-398117 FITC | 200 µg/ml | $336.00 | |||
PDPc Antibody (D-11) PE | sc-398117 PE | 200 µg/ml | $349.00 | |||
PDPc Antibody (D-11) Alexa Fluor® 488 | sc-398117 AF488 | 200 µg/ml | $364.00 | |||
PDPc Antibody (D-11) Alexa Fluor® 546 | sc-398117 AF546 | 200 µg/ml | $364.00 | |||
PDPc Antibody (D-11) Alexa Fluor® 594 | sc-398117 AF594 | 200 µg/ml | $364.00 | |||
PDPc Antibody (D-11) Alexa Fluor® 647 | sc-398117 AF647 | 200 µg/ml | $364.00 | |||
PDPc Antibody (D-11) Alexa Fluor® 680 | sc-398117 AF680 | 200 µg/ml | $364.00 | |||
PDPc Antibody (D-11) Alexa Fluor® 790 | sc-398117 AF790 | 200 µg/ml | $364.00 |