PDGF-A Antibody (E-10): sc-9974

PDGF-A Antibody (E-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects PDGF-A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. PDGF-A monoclonal antibody (E-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PDGF-A protein plays a crucial role as a mitogen for mesenchyme- and glia-derived cells, consisting of two disulfide-bonded polypeptide chains, A and B, and existing as three isoforms: PDGF AA, PDGF AB, and PDGF BB. These isoforms exhibit varying affinities for two receptor types, α and β, which are structurally related and possess protein-tyrosine kinase domains. Binding of PDGF-A to receptors induces receptor dimerization and subsequent activation of receptor kinases, with A subunit specifically binding to α receptors with high affinity, while B subunit can interact with both α and β receptors. PDGF-A has been implicated in neurotrophic functions, as evidenced by expression of receptors in oligodendrocyte progenitor cells, suggesting that PDGF-A may regulate growth and differentiation of distinct cell types in developing central nervous system through paracrine and autocrine signaling pathways.

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PDGF-A Antibody (E-10) References:

1. Assignment of interchain disulfide bonds in platelet-derived growth factor (PDGF) and evidence for agonist activity of monomeric PDGF.  |  Andersson, M., et al. 1992. J Biol Chem. 267: 11260-6. PMID: 1317862
2. Platelet-derived growth factor expression and function in idiopathic pulmonary arterial hypertension.  |  Perros, F., et al. 2008. Am J Respir Crit Care Med. 178: 81-8. PMID: 18420966
3. Initiation of oligodendrocyte progenitor cell migration by a PDGF-A activated extracellular regulated kinase (ERK) signaling pathway.  |  Frost, EE., et al. 2009. Neurochem Res. 34: 169-81. PMID: 18512152
4. Platelet-derived growth factor-alpha receptor activation is required for human cytomegalovirus infection.  |  Soroceanu, L., et al. 2008. Nature. 455: 391-5. PMID: 18701889
5. PDGF-C and -D and their receptors PDGFR-alpha and PDGFR-beta in atherosclerotic human arteries.  |  Karvinen, H., et al. 2009. Eur J Clin Invest. 39: 320-7. PMID: 19292888
6. Platelet-derived growth factor (PDGF) and PDGF receptor expression and function in folliculostellate pituitary cells.  |  Kowarik, M., et al. 2010. Exp Clin Endocrinol Diabetes. 118: 113-20. PMID: 19373754
7. PDGF-A and PDGF-B induces cardiac fibrosis in transgenic mice.  |  Gallini, R., et al. 2016. Exp Cell Res. 349: 282-290. PMID: 27816607
8. Structural characterization of the human platelet-derived growth factor A-chain cDNA and gene: alternative exon usage predicts two different precursor proteins.  |  Rorsman, F., et al. 1988. Mol Cell Biol. 8: 571-7. PMID: 2832727
9. PDGF-A suppresses contact inhibition during directional collective cell migration.  |  Nagel, M. and Winklbauer, R. 2018. Development. 145: PMID: 29884673
10. Platelet-derived growth factor A chain: gene structure, chromosomal location, and basis for alternative mRNA splicing.  |  Bonthron, DT., et al. 1988. Proc Natl Acad Sci U S A. 85: 1492-6. PMID: 3422746
11. Platelet derived growth factor-A (Pdgf-a) gene transfer modulates scar composition and improves left ventricular function after myocardial infarction.  |  Rashid, FN., et al. 2021. Int J Cardiol. 341: 24-30. PMID: 34265313
12. Role of PDGF-A/B Ligands in Cardiac Repair After Myocardial Infarction.  |  Kalra, K., et al. 2021. Front Cell Dev Biol. 9: 669188. PMID: 34513823

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PDGF-A Antibody (E-10) has 50 citations in a variety of scientific publications.

Primary antibodies like Anti-PDGF-A Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PDGF-A Antibody (E-10) Specifications

Alternate Product Name: PDGF-A

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 135-211

Epitope Target: PDGF-A

Epitope Species: human

Product Reactivity/Detection: precursor and mature PDGF-A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 50 citations in various scientific publications

Available Conjugates/Forms for PDGF-A Antibody (E-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin