PDGF-A Antibody (A-1): sc-390392

PDGF-A Antibody (A-1) is a mouse monoclonal IgG1 antibody that detects PDGF-A in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PDGF-A plays a crucial role as a mitogen for mesenchyme- and glia-derived cells, consisting of two disulfide-bonded polypeptide chains, A and B, and existing in three isoforms: PDGF AA, PDGF AB, and PDGF BB. These isoforms exhibit varying affinities for two receptor types, α and β, which are structurally related and possess protein-tyrosine kinase domains. The binding of PDGF-A to its receptors induces receptor dimerization and subsequent activation of the receptor kinases, with the A subunit specifically binding to α receptors with high affinity, while the B subunit can interact with both α and β receptors. Notably, PDGF-A has been implicated as a neurotrophic factor, with evidence indicating that its receptors are expressed in oligodendrocyte progenitor cells, while PDGF-B receptors are found on neurons. This suggests that the distinct isoforms of PDGF may play a pivotal role in regulating the growth and differentiation of various cell types in the developing central nervous system through paracrine and autocrine signaling pathways. Anti-PDGF-A antibody (A-1) is an essential tool for researchers investigating the complex roles of PDGF-A in cellular processes and its implications in neurodevelopment.

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PDGF-A Antibody (A-1) References:

1. PDGF-A/PDGF alpha-receptor signaling is required for lung growth and the formation of alveoli but not for early lung branching morphogenesis.  |  Boström, H., et al. 2002. Dev Dyn. 223: 155-62. PMID: 11803579
2. PDGF-A, PDGF-B, TGFbeta, and bFGF mRNA levels in patients with essential thrombocythemia treated with anagrelide.  |  Lev, PR., et al. 2005. Am J Hematol. 78: 155-7. PMID: 15682418
3. Puralpha activates PDGF-A gene transcription via interactions with a G-rich, single-stranded region of the promoter.  |  Zhang, Q., et al. 2005. Gene. 348: 25-32. PMID: 15777709
4. PDGF-A, -C, and -D but not PDGF-B increase TGF-beta1 and chronic rejection in rat cardiac allografts.  |  Tuuminen, R., et al. 2009. Arterioscler Thromb Vasc Biol. 29: 691-8. PMID: 19213942
5. PDGF-Ralpha gene expression predicts proliferation, but PDGF-A suppresses transdifferentiation of neonatal mouse lung myofibroblasts.  |  Kimani, PW., et al. 2009. Respir Res. 10: 119. PMID: 19939260
6. The increased PDGF-A, PDGF-B and FGF-2 expression in recurrence of salivary gland pleomorphic adenoma.  |  Soares, AB., et al. 2012. J Clin Pathol. 65: 272-7. PMID: 22174424
7. PDGF-A signaling is required for secondary alveolar septation and controls epithelial proliferation in the developing lung.  |  Gouveia, L., et al. 2018. Development. 145: PMID: 29636361
8. Role of PDGF-A-Activated ERK Signaling Mediated FAK-Paxillin Interaction in Oligodendrocyte Progenitor Cell Migration.  |  Singh, J., et al. 2019. J Mol Neurosci. 67: 564-573. PMID: 30649683
9. Accumulation of PDGF B and cell-binding forms of PDGF A in the extracellular matrix.  |  Kelly, JL., et al. 1993. J Cell Biol. 121: 1153-63. PMID: 8501120
10. Not all myofibroblasts are alike: revisiting the role of PDGF-A and PDGF-B using PDGF-targeted mice.  |  Lindahl, P. and Betsholtz, C. 1998. Curr Opin Nephrol Hypertens. 7: 21-6. PMID: 9442358

The preferred PDGF-A antibody is PDGF-A Antibody (E-10): sc-9974.