PDE4A Antibody (H-7): sc-74428

PDE4A Antibody (H-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects PDE4A protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-PDE4A antibody (H-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PDE4A plays a crucial role in regulating intracellular cyclic adenosine monophosphate (cAMP) levels, which are vital for numerous cellular processes, including cell signaling, metabolism, and gene expression. By hydrolyzing cAMP to 5′-adenosine monophosphate (5′-AMP), PDE4A helps maintain cAMP balance, thereby influencing pathways that control inflammation, neuronal signaling, and cardiac function. The human PDE4A gene, located on chromosome 19p13.2, spans 50 kilobases and consists of 17 exons, producing several splice variants, including isoforms PDE4A1-6. Notably, the PDE4 family is characterized by a unique upstream conserved region (UCR) that is essential for dimerization of isoforms, highlighting the complexity and regulatory potential in various physiological contexts.

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PDE4A Antibody (H-7) References:

1. Physical mapping and promoter structure of the murine cAMP-specific phosphodiesterase pde4a gene.  |  Olsen, AE. and Bolger, GB. 2000. Mamm Genome. 11: 41-5. PMID: 10602991
2. Cyclic nucleotide phosphodiesterases: relating structure and function.  |  Francis, SH., et al. 2001. Prog Nucleic Acid Res Mol Biol. 65: 1-52. PMID: 11008484
3. Dimerization of the type 4 cAMP-specific phosphodiesterases is mediated by the upstream conserved regions (UCRs).  |  Richter, W. and Conti, M. 2002. J Biol Chem. 277: 40212-21. PMID: 12177055
4. Role of AKAP 149-PKA-PDE4A complex in cell survival and cell differentiation processes.  |  Fernández-Araujo, A., et al. 2014. Int J Biochem Cell Biol. 53: 89-101. PMID: 24813785
5. PDE4a predicts poor prognosis and promotes metastasis by inducing epithelial-mesenchymal transition in hepatocellular carcinoma.  |  Peng, Y., et al. 2018. J Cancer. 9: 2389-2396. PMID: 30026835
6. Overexpression of PDE4A Acts as Checkpoint Inhibitor Against cAMP-Mediated Immunosuppression in vitro.  |  Schmetterer, KG., et al. 2019. Front Immunol. 10: 1790. PMID: 31417563
7. CircAXL Knockdown Alleviates Aβ1-42-Induced Neurotoxicity in Alzheimer's Disease via Repressing PDE4A by Releasing miR-1306-5p.  |  Meng, S., et al. 2022. Neurochem Res. 47: 1707-1720. PMID: 35229272
8. Low PDE4A expression promoted the progression of ovarian cancer by inducing Snail nuclear translocation.  |  Wang, J., et al. 2024. Exp Cell Res. 439: 114100. PMID: 38797258
9. Molecular cloning of a novel splice variant of human type IVA (PDE-IVA) cyclic AMP phosphodiesterase and localization of the gene to the p13.2-q12 region of human chromosome 19 [corrected].  |  Horton, YM., et al. 1995. Biochem J. 308 (Pt 2): 683-91. PMID: 7772058
10. A family of human phosphodiesterases homologous to the dunce learning and memory gene product of Drosophila melanogaster are potential targets for antidepressant drugs.  |  Bolger, G., et al. 1993. Mol Cell Biol. 13: 6558-71. PMID: 8413254
11. Intracellular targeting, interaction with Src homology 3 (SH3) domains and rolipram-detected conformational switches in cAMP-specific PDE4A phosphodiesterase.  |  Houslay, MD., et al. 1997. Biochem Soc Trans. 25: 374-81. PMID: 9191121
12. Zinc dependent activation of cAMP-specific phosphodiesterase (PDE4A).  |  Percival, MD., et al. 1997. Biochem Biophys Res Commun. 241: 175-80. PMID: 9405253

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PDE4A Antibody (H-7) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-PDE4A Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PDE4A Antibody (H-7) Specifications

Alternate Product Name: PDE4A

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 721-886

Epitope Target: PDE4A

Epitope Species: human

Product Reactivity/Detection: PDE4A isoforms 1-5

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for PDE4A Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin