PDE11A Antibody (B-7): sc-365655
- PDE11A Antibody (B-7) is a mouse monoclonal IgG1 κ PDE11A antibody provided at 200 µg/ml
- raised against amino acids 373-535 mapping within the GAF1 domain of PDE11A of human origin
- PDE11A Antibody (B-7) is recommended for detection of PDE11A of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PDE11A Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PDE11A Antibody (B-7) for WB applications. These reagents are now offered in bundles with PDE11A Antibody (B-7) (see ordering information below).
PDE11A Antibody (B-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects PDE11A of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PDE11A antibody (B-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PDE11A, also known as phosphodiesterase 11A or PPNAD2, is a crucial enzyme that plays a significant role in regulating cyclic nucleotide signaling by hydrolyzing cAMP and cGMP into their inactive forms, 5′-AMP and 5′-GMP, respectively. This enzymatic activity maintains cellular homeostasis and modulates various physiological processes, including hormone signaling and neuronal communication. PDE11A is expressed in multiple tissues and exists in four isoforms, each exhibiting distinct tissue specificity, which underscores its importance in diverse biological contexts. PDE11A contains two GAF domains that facilitate catalytic function and is regulated by free cGMP binding, which acts as an allosteric activator, enhancing enzymatic activity. Dysregulation or defects in PDE11A can lead to primary pigmented nodular adrenocortical disease type 2 (PPNAD2), a condition associated with endocrine-related Cushing syndrome, highlighting clinical relevance in endocrine disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PDE11A Antibody (B-7) References:
- Cloning and characterization of two splice variants of human phosphodiesterase 11A. | Hetman, JM., et al. 2000. Proc Natl Acad Sci U S A. 97: 12891-5. PMID: 11050148
- 3',5'-cyclic nucleotide phosphodiesterase 11A: localization in human tissues. | Loughney, K., et al. 2005. Int J Impot Res. 17: 320-5. PMID: 15800651
- Expression of PDE11A in normal and malignant human tissues. | D'Andrea, MR., et al. 2005. J Histochem Cytochem. 53: 895-903. PMID: 15995148
- cAMP is a ligand for the tandem GAF domain of human phosphodiesterase 10 and cGMP for the tandem GAF domain of phosphodiesterase 11. | Gross-Langenhoff, M., et al. 2006. J Biol Chem. 281: 2841-6. PMID: 16330539
- A genome-wide scan identifies mutations in the gene encoding phosphodiesterase 11A4 (PDE11A) in individuals with adrenocortical hyperplasia. | Horvath, A., et al. 2006. Nat Genet. 38: 794-800. PMID: 16767104
- Phosphodiesterase genes are associated with susceptibility to major depression and antidepressant treatment response. | Wong, ML., et al. 2006. Proc Natl Acad Sci U S A. 103: 15124-9. PMID: 17008408
- Adrenal hyperplasia and adenomas are associated with inhibition of phosphodiesterase 11A in carriers of PDE11A sequence variants that are frequent in the population. | Horvath, A., et al. 2006. Cancer Res. 66: 11571-5. PMID: 17178847
- The properties of phosphodiesterase 11A4 GAF domains are regulated by modifications in its N-terminal domain. | Gross-Langenhoff, M., et al. 2008. FEBS J. 275: 1643-50. PMID: 18312413
- Phosphodiesterase 11A (PDE11A) and genetic predisposition to adrenocortical tumors. | Libé, R., et al. 2008. Clin Cancer Res. 14: 4016-24. PMID: 18559625
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDE11A Antibody (B-7) | sc-365655 | 200 µg/ml | $316.00 | |||
PDE11A Antibody (B-7): m-IgG Fc BP-HRP Bundle | sc-529427 | 200 µg Ab; 10 µg BP | $354.00 | |||
PDE11A Antibody (B-7): m-IgGκ BP-HRP Bundle | sc-522315 | 200 µg Ab, 40 µg BP | $354.00 | |||
PDE11A Antibody (B-7): m-IgG1 BP-HRP Bundle | sc-543512 | 200 µg Ab; 20 µg BP | $354.00 | |||
PDE11A Antibody (B-7) AC | sc-365655 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PDE11A Antibody (B-7) HRP | sc-365655 HRP | 200 µg/ml | $316.00 | |||
PDE11A Antibody (B-7) FITC | sc-365655 FITC | 200 µg/ml | $330.00 | |||
PDE11A Antibody (B-7) PE | sc-365655 PE | 200 µg/ml | $343.00 | |||
PDE11A Antibody (B-7) Alexa Fluor® 488 | sc-365655 AF488 | 200 µg/ml | $357.00 | |||
PDE11A Antibody (B-7) Alexa Fluor® 546 | sc-365655 AF546 | 200 µg/ml | $357.00 | |||
PDE11A Antibody (B-7) Alexa Fluor® 594 | sc-365655 AF594 | 200 µg/ml | $357.00 | |||
PDE11A Antibody (B-7) Alexa Fluor® 647 | sc-365655 AF647 | 200 µg/ml | $357.00 | |||
PDE11A Antibody (B-7) Alexa Fluor® 680 | sc-365655 AF680 | 200 µg/ml | $357.00 | |||
PDE11A Antibody (B-7) Alexa Fluor® 790 | sc-365655 AF790 | 200 µg/ml | $357.00 |