CD274/PD-L1 Antibody (D-8): sc-518027

Pdcd-1L1 Antibody (D-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects Pdcd-1L1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Pdcd-1L1 (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Pdcd-1L1, also known as programmed cell death ligand-1 or B7-H1, is a crucial type I transmembrane protein composed of 290 amino acids, which plays a significant role in immune regulation by interacting with PD-1 receptor on T cells, thereby inhibiting T cell activation and promoting immune tolerance. This interaction is particularly important in cancer, where tumor cells often exploit Pdcd-1L1 pathway to evade immune detection, making Pdcd-1L1 a key target for immunotherapy. Pdcd-1L1 features immunoglobulin V-like and C-like domains, along with a 30 amino acid cytoplasmic tail, and is expressed constitutively on various tissues, including heart, lung, and kidney, suggesting involvement in maintaining immune homeostasis and preventing autoimmune responses. The gene encoding Pdcd-1L1 is located on human chromosome 9p24, and Pdcd-1L1 expression is essential for modulating immune response, particularly in tumor microenvironment, where Pdcd-1L1 can influence efficacy of T cell-mediated anti-tumor immunity.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD274/PD-L1 Antibody (D-8) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-CD274/PD-L1 Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD274/PD-L1 Antibody (D-8) Specifications

Alternate Product Name: PD-L1

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 221-240

Epitope Target: PD-L1

Epitope Species: human

Product Reactivity/Detection: PD-L1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for CD274/PD-L1 Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD274/PD-L1 antibody is CD274/PD-L1 Antibody (1C10): sc-293425.