PCP-2 Antibody (F-3): sc-137064

PCP-2 Antibody (F-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects PCP-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PCP-2 (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Purkinje cell protein-2 (PCP-2), also known as L7, is a crucial G protein regulator predominantly found in Purkinje cells and retinal bipolar neurons, playing a significant role in modulating synaptic activity and neural differentiation. PCP-2 is characterized by its GoLoco or GPR motif, which is essential for interaction with the Gαi/o family of G proteins, effectively inhibiting GDP release. This interaction suggests that Gαi/o and PCP-2 colocalization in the cerebellum may influence synaptic function and neuronal development. The physiological effectors of PCP-2 include Purkinje-type calcium channels, which are the primary voltage-dependent channels regulating neuronal output and are modulated by Gi/o proteins. PCP-2 is exclusively detected in higher vertebrates, indicating potential as a marker for evolutionary advancement of cerebellar Purkinje cells, thus underscoring importance in understanding both normal and pathological neural processes.

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PCP-2 Antibody (F-3) References:

1. Conservation of the developmentally regulated dendritic localization of a Purkinje cell-specific mRNA that encodes a G-protein modulator: comparison of rodent and human Pcp2(L7) gene structure and expression.  |  Zhang, X., et al. 2002. Brain Res Mol Brain Res. 105: 1-10. PMID: 12399102
2. Association and colocalization of G protein alpha subunits and Purkinje cell protein 2 (Pcp2) in mammalian cerebellum.  |  Redd, KJ., et al. 2002. J Neurosci Res. 70: 631-7. PMID: 12424730
3. Genomic structure and alternative splicing of murine R2B receptor protein tyrosine phosphatases (PTPkappa, mu, rho and PCP-2).  |  Besco, J., et al. 2004. BMC Genomics. 5: 14. PMID: 15040814
4. A Purkinje cell specific GoLoco domain protein, L7/Pcp-2, modulates receptor-mediated inhibition of Cav2.1 Ca2+ channels in a dose-dependent manner.  |  Kinoshita-Kawada, M., et al. 2004. Brain Res Mol Brain Res. 132: 73-86. PMID: 15548431
5. Identification of candidate Purkinje cell-specific markers by gene expression profiling in wild-type and pcd(3J) mice.  |  Rong, Y., et al. 2004. Brain Res Mol Brain Res. 132: 128-45. PMID: 15582153
6. L7/Pcp-2-specific expression of Cre recombinase using knock-in approach.  |  Saito, H., et al. 2005. Biochem Biophys Res Commun. 331: 1216-21. PMID: 15883005
7. Enhanced Purkinje cell survival but compromised cerebellar function in targeted anti-apoptotic protein transgenic mice.  |  Goswami, J., et al. 2005. Mol Cell Neurosci. 29: 202-21. PMID: 15911345
8. Purkinje cell protein-2 (Pcp2) stimulates differentiation in PC12 cells by Gbetagamma-mediated activation of Ras and p38 MAPK.  |  Guan, J., et al. 2005. Biochem J. 392: 389-97. PMID: 15948714
9. G-protein alpha subunit interaction and guanine nucleotide dissociation inhibitor activity of the dual GoLoco motif protein PCP-2 (Purkinje cell protein-2).  |  Willard, FS., et al. 2006. Cell Signal. 18: 1226-34. PMID: 16298104
10. Genomics, morphogenesis and biophysics: triangulation of Purkinje cell development.  |  Simons, MJ. and Pellionisz, AJ. 2006. Cerebellum. 5: 27-35. PMID: 16527761
11. Protein-tyrosine phosphatase PCP-2 inhibits beta-catenin signaling and increases E-cadherin-dependent cell adhesion.  |  Yan, HX., et al. 2006. J Biol Chem. 281: 15423-33. PMID: 16574648
12. The carboxypeptidase-like substrate-binding site in Nna1 is essential for the rescue of the Purkinje cell degeneration (pcd) phenotype.  |  Wang, T., et al. 2006. Mol Cell Neurosci. 33: 200-13. PMID: 16952463

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PCP-2 Antibody (F-3) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-PCP-2 Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PCP-2 Antibody (F-3) Specifications

Alternate Product Name: PCP-2

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-60

Epitope Target: PCP-2

Epitope Species: human

Product Reactivity/Detection: PCP-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for PCP-2 Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin