PCNA Antibody (PC11) recommended for detection of PCNA of mouse, rat and human origin by WB, IP, IF and IHC(P)

PCNA Antibody (PC11): sc-53407

PCNA Antibody (PC11) is rated 4.0 out of 5 by 5.
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Datasheets

    • PCNA Antibody (PC11) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against protein A-A-PCNA fusion obtained from pC2T cells
    • recommended for detection of PCNA of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See PCNA (PC10): sc-56 for PCNA antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

I am using PCNA (PC11): sc-53407 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Strong WB band in human 293T whole cell lysates Strong WB band in human 293T whole cell lysates. -SCBT QC
Date published: 2015-05-02
Rated 4 out of 5 by from Good specificity in WB and IHC in human Muscularis Good specificity in WB and IHC in human Muscularis mucosa samples. -SCBT Publication Review
Date published: 2015-03-27
Rated 4 out of 5 by from Worked well in WB in A Worked well in WB in A-431, MOLT-4 whole cell lysates and Hel G2, HeLa, CCRF-CEM nuclear extracts. -SCBT QC
Date published: 2015-03-07
Rated 4 out of 5 by from Good staining in IHC in rat mammary tumors Good staining in IHC in rat mammary tumors, ovary, kidney, uterus, lung and liver tissues. -SCBT Publication Review
Date published: 2015-01-09
Rated 4 out of 5 by from Strong nuclear localization in IF in methanol Strong nuclear localization in IF in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-07
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