Anti-PCNA Antibody (PC10) recommended for detection of PCNA p36 protein expressed at high levels in proliferating cells of mouse, rat, human, insect and S. pombe origin by WB, IP, IF, IHC(P) and FCM

Anti-PCNA Antibody (PC10): sc-56

Anti-PCNA Antibody (PC10) is rated 4.8 out of 5 by 110.
  • y_2022, m_8, d_8, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.32
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_109
  • loc_en_US, sid_56, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 38ms
  • REVIEWS, PRODUCT
loading
5
98
4
9
3
2
2
0
1
1

Datasheets

    • Anti-PCNA Antibody (PC10) is a mouse monoclonal IgG2a κ PCNA antibody, cited in 2,784 publications, provided at 200 µg/ml
    • raised against rat PCNA made in the protein A expression vector pR1T2T
    • Anti-PCNA Antibody (PC10) is recommended for detection of PCNA p36 protein expressed at high levels in proliferating cells of mouse, rat, human, insect and S. pombe origin by WB, IP, IF, IHC(P) and FCM
    • Anti-PCNA Antibody (PC10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA; and to either TRITC, PerCP or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PCNA (PC10): sc-56.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for PCNA Antibody (PC10) for WB and IHC(P) applications. These reagents are now offered in bundles with PCNA Antibody (PC10) (see ordering information below).
loading

does antibody m-IgGκ BP-HRP:(sc-516102) compatible to using as secondary antibody against Anti-PCNA Antibody (PC10): sc-56 for western blot

Asked by: Anonymous
Thank you for your question. Anti-PCNA Antibody (PC10) is a mouse monoclonal IgG2a κ PCNA antibody, so it is compatible with m-IgGκ BP-HRP:(sc-516102), which recognizes kappa light chain mouse IgG, IgA and IgM antibodies.
Answered by: Tech Support Europe
Date published: 2021-06-25

Recently we observed that the PCNA sc-56 antibody in the tube is precipitated. It was stored at 4 degrees as recommended. Can the antibody be used for experimental purpose? If yes how do we use it? any recommendation from company's side?

Asked by: Anonymous
Thank you for your question. Please contact technical service directly so they can look into this matter.
Answered by: Tech Support Europe
Date published: 2021-01-18

Which antigen retrieval buffer should be used with PCNA sc-56, citrate or Tris? Can you please include this information in the product datasheet so that it becomes convenient for the user to begin with.

Asked by: Sobia
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval with this PCNA antibody. The detailed method can be found in our recommended protocol online.
Answered by: Technical Support
Date published: 2019-11-22

Can I use this mouse monoclonal antibody for immunohistochemical staining of swine(pig) tissue?

Asked by: xgtian
Thank you for your question. We have not tested this antibody on swine or pig tissue, however, we are aware of a few publications citing use of this antibody in pig samples. For example, PMID: 26389662.
Answered by: Technical Support
Date published: 2019-07-13

We are new to IHC. Thinking of using this antibody, PCNA Antibody: sc-56on our chicken samples. Wondering if there are any preferences on the conjugate for the secondary antibody, such as HRP, biotin etc. We don't need fluorescence

Asked by: SDas
Thank you for your question. This antibody has been previously cited for detection of PCNA in chicken samples. For IHC, you could use the unconjugated antibody, with an appropriate secondary antibody for detection, or the Biotin conjugated primary with an ABC Staining System, or the HRP conjugate for direct immunoperoxidase staining. Using the directly conjugated primary antibody can save you time and money on reagents. We do not have a preference for this particular antibody, and think you would be successful using either version of the antibody, depending on your preferences in your lab.
Answered by: Technical Support
Date published: 2019-06-22

PCNA Antibody (PC10) how much of 200ug/ml of this antibody I am going to get in one order?

Asked by: Anukana
Thank you for your question. This is provided as 200 ug in 1 mL.
Answered by: Technical Support
Date published: 2020-01-15

I'm wondering about the region of PCNA that is recognized by this antibody SC-56, PC10? Would an N-terminal Flag tag get in the way? Thank you.

Asked by: Galyah
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not been determined. However, some report that this clone (PC10) recognizes an epitope within the amino acid range of 111-125. In that case, we would not expect an N-terminal Flag tag to prevent this antibody from recognizing PCNA that contains this sequence.
Answered by: Technical Support
Date published: 2019-03-21

Sir, I want to get free sample of PCNA Antibody (PC10): sc-56. Please update me with the process to avail. Thank you.

Asked by: Pinky Kaur
Thank you for your question. Please contact your local distributor for more information on how to receive a free 10 ug sample of this monoclonal antibody
Answered by: Tech Service
Date published: 2019-03-13
  • y_2022, m_8, d_8, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.32
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_13
  • loc_en_US, sid_56, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 17ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Works great for IF with super-resolution imaging I got the Alexa-488 conjugated antibody and have used to for IF on U-2 OS cells. There was nuclear specific PCNA labelling. I imaged the samples with dSTORM and SIM methods and the immunolabeling looks great.
Date published: 2021-07-29
Rated 5 out of 5 by from Works very well! We blotted several times with this antibody and it works perfectly!
Date published: 2021-06-16
Rated 5 out of 5 by from PCNA Ab for WB and IF Excellent antibody for WB and IF (fixed with paraformaldehyde) dilution 1:2000 Good housekeeping protein for human colon cancer cell lines
Date published: 2021-03-23
Rated 5 out of 5 by from Excellent antibody Excellent bands with high dilution and no background
Date published: 2021-03-17
Rated 5 out of 5 by from SIRTs inhibitors and sorafenib SIRTs inhibitors lead to a diminution of cellular proliferation during sorafenib treatment
Date published: 2021-02-26
Rated 5 out of 5 by from Immunohistochemistryで使用しました。 マウス脳切片を用いて、Immunohistochemistryで使用しました。感度よく検出できたと思います。
Date published: 2020-08-05
Rated 1 out of 5 by from 做核质分离竟然只在胞质有??? 去年买了这个抗体,做核质分离竟然只在胞质有???同时用了GAPDH和Lanmin A,证实了这个抗体只在胞质,太坑人了
Date published: 2020-07-01
Rated 5 out of 5 by from 免疫染色に優れています。 HCT116細胞を4%PFAで固定し、この抗体を200倍希釈で使用しました。免疫染色ではきれいな結果が得られました。PCNA(F-2)に関しも、同様に良い結果が得られました。
Date published: 2020-03-28
  • y_2022, m_8, d_8, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.32
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_109
  • loc_en_US, sid_56, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT