PCNA Antibody (6D645) recommended for detection of PCNA p36 protein expressed at high levels in proliferating cells of mouse, rat, human, insect and S. pombe origin by WB, IP, IF, IHC(P) and FCM

PCNA Antibody (6D645): sc-71858

PCNA Antibody (6D645) is rated 4.0 out of 5 by 2.
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Datasheets

    • PCNA Antibody (6D645) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against rat PCNA made in the protein A expression vector pR1T2T
    • recommended for detection of PCNA p36 protein expressed at high levels in proliferating cells of mouse, rat, human, insect and S. pombe origin by WB, IP, IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See PCNA (PC10): sc-56 for PCNA antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Can PCNA (6D645): sc-71858 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Dr Ninau Qelp
Thank you for your question. Yes, PCNA (6D645): sc-71858 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from Worked well in WB in mouse 293T and A Worked well in WB in mouse 293T and A-431 whole cell lysates. -SCBT QC
Date published: 2015-02-12
Rated 4 out of 5 by from Strong band in WB in human 293T whole cell lysates Strong band in WB in human 293T whole cell lysates. -SCBT QC
Date published: 2013-09-14
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