PCM1 Antibody (G-6): sc-398365
- PCM1 Antibody (G-6) is a mouse monoclonal IgG1 κ PCM1 antibody, cited in 35 publications, provided at 200 µg/ml
- raised against amino acids 1-262 mapping at the N-terminus of PCM1 of human origin
- PCM1 Antibody (G-6) is recommended for detection of PCM1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PCM1 Antibody (G-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PCM1 Antibody (G-6) for WB applications. These reagents are now offered in bundles with PCM1 Antibody (G-6) (see ordering information below).
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PCM1 (G-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects PCM1 protein of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). PCM1 (G-6) antibody is available as both non-conjugated form, as well as multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. Pericentriolar Material is a dynamic substance whose composition oscillates during the cell cycle. PCM1 (Pericentriolar Material 1) is a centrosomal protein showing distinct cell cycle-dependent association with the centrosome complex. PCM1 (G-6) monoclonal antibody associates securely with the centrosome complex through G1, S, and part of G2. Late in G2, as cells prepare for mitosis, PCM1 dissociates from the centrosome and remains evenly diffused throughout the cell during mitosis before re-associating with centrosomes in G1 phase progeny cells. PCM1′s chromosomal location on 8p22-p21.3 draws interest since this region commonly shows deletions in several tumors. In thyroid tumor tissue, PCM1 expression significantly decreases with altered subcellular localization.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PCM1 Antibody (G-6) References:
- RET/PCM-1: a novel fusion gene in papillary thyroid carcinoma. | Corvi, R., et al. 2000. Oncogene. 19: 4236-42. PMID: 10980597
- Dynamic recruitment of Nek2 kinase to the centrosome involves microtubules, PCM-1, and localized proteasomal degradation. | Hames, RS., et al. 2005. Mol Biol Cell. 16: 1711-24. PMID: 15659651
- The t(8;9)(p22;p24) is a recurrent abnormality in chronic and acute leukemia that fuses PCM1 to JAK2. | Reiter, A., et al. 2005. Cancer Res. 65: 2662-7. PMID: 15805263
- Complex CGH alterations on chromosome arm 8p at candidate tumor suppressor gene loci in breast cancer cell lines. | Venter, DJ., et al. 2005. Cancer Genet Cytogenet. 160: 134-40. PMID: 15993269
- JAK the trigger. | Mahon, FX. 2005. Oncogene. 24: 7125-6. PMID: 16007127
- PCM1-JAK2 fusion in myeloproliferative disorders and acute erythroid leukemia with t(8;9) translocation. | Murati, A., et al. 2005. Leukemia. 19: 1692-6. PMID: 16034466
- The t(8;9)(p22;p24) translocation in atypical chronic myeloid leukaemia yields a new PCM1-JAK2 fusion gene. | Bousquet, M., et al. 2005. Oncogene. 24: 7248-52. PMID: 16091753
- PCM1-JAK2-fusion: a potential treatment target in myelodysplastic-myeloproliferative and other hemato-lymphoid neoplasms. | Hoeller, S., et al. 2011. Expert Opin Ther Targets. 15: 53-62. PMID: 21091042
- PCM1 labeling reveals myonuclear and nuclear dynamics in skeletal muscle across species. | Viggars, MR., et al. 2023. Am J Physiol Cell Physiol. 324: C85-C97. PMID: 36409178
- Navigating centriolar satellites: the role of PCM1 in cellular and organismal processes. | Begar, E., et al. 2024. FEBS J.. PMID: 38825736
- Mapping of the human autoantigen pericentriolar material 1 (PCM1) gene to chromosome 8p21.3-p22. | Ohata, H., et al. 1994. Genomics. 24: 404-6. PMID: 7698772
- PCM-1, A 228-kD centrosome autoantigen with a distinct cell cycle distribution. | Balczon, R., et al. 1994. J Cell Biol. 124: 783-93. PMID: 8120099
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PCM1 Antibody (G-6) | sc-398365 | 200 µg/ml | $316.00 | |||
PCM1 Antibody (G-6): m-IgG Fc BP-HRP Bundle | sc-530774 | 200 µg Ab; 10 µg BP | $354.00 | |||
PCM1 Antibody (G-6): m-IgGκ BP-HRP Bundle | sc-524311 | 200 µg Ab, 40 µg BP | $354.00 | |||
PCM1 Antibody (G-6): m-IgG1 BP-HRP Bundle | sc-544246 | 200 µg Ab; 20 µg BP | $354.00 | |||
PCM1 Antibody (G-6) AC | sc-398365 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PCM1 Antibody (G-6) HRP | sc-398365 HRP | 200 µg/ml | $316.00 | |||
PCM1 Antibody (G-6) FITC | sc-398365 FITC | 200 µg/ml | $330.00 | |||
PCM1 Antibody (G-6) PE | sc-398365 PE | 200 µg/ml | $343.00 | |||
PCM1 Antibody (G-6) Alexa Fluor® 488 | sc-398365 AF488 | 200 µg/ml | $357.00 | |||
PCM1 Antibody (G-6) Alexa Fluor® 546 | sc-398365 AF546 | 200 µg/ml | $357.00 | |||
PCM1 Antibody (G-6) Alexa Fluor® 594 | sc-398365 AF594 | 200 µg/ml | $357.00 | |||
PCM1 Antibody (G-6) Alexa Fluor® 647 | sc-398365 AF647 | 200 µg/ml | $357.00 | |||
PCM1 Antibody (G-6) Alexa Fluor® 680 | sc-398365 AF680 | 200 µg/ml | $357.00 | |||
PCM1 Antibody (G-6) Alexa Fluor® 790 | sc-398365 AF790 | 200 µg/ml | $357.00 |