PCLAF Antibody (G-11): sc-390515
- PCLAF Antibody (G-11) is a mouse monoclonal IgG2a κ PCLAF antibody, cited in 11 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 58-78 of PCLAF of mouse origin
- PCLAF Antibody (G-11) is recommended for detection of PCLAF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-PCLAF Antibody (G-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PCLAF Antibody (G-11) for WB and IHC(P) applications. These reagents are now offered in bundles with PCLAF Antibody (G-11) (see ordering information below).
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PCLAF Antibody (G-11) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect PCLAF, also known as p15 (PAF), KIAA0101, PAF15, and PCNA-associated factor of 15 kDa. PCLAF monoclonal antibody (G-11) targets a precise epitope spanning amino acids 58-78 of mouse PCLAF, ensuring accurate and reliable detection in various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) across mouse, rat, and human samples. PCLAF is composed of 111 amino acids and possesses a well-defined structure crucial for interaction with proliferating cell nuclear antigen (PCNA), a key player in DNA replication and repair processes. This structural configuration allows PCLAF to effectively bind PCNA, thereby playing a vital role in protecting cells from UV-induced apoptosis by facilitating DNA damage tolerance mechanisms. Additionally, PCLAF′s structural integrity enables interaction with tumor suppressor proteins such as ING1, which influences apoptotic pathways and cell cycle regulation. PCLAF′s importance is further highlighted by elevated expression in various cancers, including esophageal and pancreatic tumors, where anti-PCLAF antibody (G-11) can help investigate contributions to tumor progression through cell survival and proliferation modulation. PCLAF (G-11) antibody serves as an essential tool for studies focused on cell cycle regulation and tumorigenesis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PCLAF Antibody (G-11) References:
- p15(PAF), a novel PCNA associated factor with increased expression in tumor tissues. | Yu, P., et al. 2001. Oncogene. 20: 484-9. PMID: 11313979
- Overexpressed in anaplastic thyroid carcinoma-1 (OEATC-1) as a novel gene responsible for anaplastic thyroid carcinoma. | Mizutani, K., et al. 2005. Cancer. 103: 1785-90. PMID: 15789362
- The PCNA-associated factor KIAA0101/p15(PAF) binds the potential tumor suppressor product p33ING1b. | Simpson, F., et al. 2006. Exp Cell Res. 312: 73-85. PMID: 16288740
- KIAA0101 (OEACT-1), an expressionally down-regulated and growth-inhibitory gene in human hepatocellular carcinoma. | Guo, M., et al. 2006. BMC Cancer. 6: 109. PMID: 16646990
- Murine embryonic expression of the gene for the UV-responsive protein p15(PAF). | van Bueren, KL., et al. 2007. Gene Expr Patterns. 7: 47-50. PMID: 16815099
- Oncogenic role of KIAA0101 interacting with proliferating cell nuclear antigen in pancreatic cancer. | Hosokawa, M., et al. 2007. Cancer Res. 67: 2568-76. PMID: 17363575
- Overexpression of KIAA0101 predicts high stage, early tumor recurrence, and poor prognosis of hepatocellular carcinoma. | Yuan, RH., et al. 2007. Clin Cancer Res. 13: 5368-76. PMID: 17875765
- NS5ATP9, a gene up-regulated by HCV NS5A protein. | Shi, L., et al. 2008. Cancer Lett. 259: 192-7. PMID: 18068894
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PCLAF Antibody (G-11) | sc-390515 | 200 µg/ml | $322.00 | |||
PCLAF Antibody (G-11): m-IgG Fc BP-HRP Bundle | sc-530177 | 200 µg Ab; 10 µg BP | $361.00 | |||
PCLAF Antibody (G-11): m-IgGκ BP-HRP Bundle | sc-523567 | 200 µg Ab, 40 µg BP | $361.00 | |||
PCLAF Antibody (G-11) AC | sc-390515 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PCLAF Antibody (G-11) HRP | sc-390515 HRP | 200 µg/ml | $322.00 | |||
PCLAF Antibody (G-11) FITC | sc-390515 FITC | 200 µg/ml | $336.00 | |||
PCLAF Antibody (G-11) PE | sc-390515 PE | 200 µg/ml | $349.00 | |||
PCLAF Antibody (G-11) Alexa Fluor® 488 | sc-390515 AF488 | 200 µg/ml | $364.00 | |||
PCLAF Antibody (G-11) Alexa Fluor® 546 | sc-390515 AF546 | 200 µg/ml | $364.00 | |||
PCLAF Antibody (G-11) Alexa Fluor® 594 | sc-390515 AF594 | 200 µg/ml | $364.00 | |||
PCLAF Antibody (G-11) Alexa Fluor® 647 | sc-390515 AF647 | 200 µg/ml | $364.00 | |||
PCLAF Antibody (G-11) Alexa Fluor® 680 | sc-390515 AF680 | 200 µg/ml | $364.00 | |||
PCLAF Antibody (G-11) Alexa Fluor® 790 | sc-390515 AF790 | 200 µg/ml | $364.00 | |||
PCLAF (G-11) Neutralizing Peptide | sc-390515 P | 100 µg/0.5 ml | $69.00 |