PCAF Antibody (E-8): sc-13124
- PCAF Antibody (E-8) is a mouse monoclonal IgG1 κ PCAF antibody, cited in 111 publications, provided at 200 µg/ml
- raised against amino acids 464-832 of PCAF of human origin
- PCAF Antibody (E-8) is recommended for detection of PCAF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; non cross-reactive with GCN5
- Anti-PCAF Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PCAF Antibody (E-8) for WB and IHC(P) applications. These reagents are now offered in bundles with PCAF Antibody (E-8) (see ordering information below).
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PCAF Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 464-832 of human PCAF, also known as P300/CBP-associated factor, KAT2B, or P/CAF. PCAF is a histone acetyltransferase (HAT) that plays a pivotal role in regulating gene expression by acetylating lysine residues on histone proteins, leading to chromatin remodeling and transcriptional activation. This modification relaxes the chromatin structure, allowing transcription factors easier access to DNA and thereby influencing crucial cellular processes such as cell cycle progression, differentiation, and response to external signals. PCAF also acetylates non-histone proteins, affecting their function and stability, which underscores its importance in various cellular pathways. PCAF (E-8) antibody is specific for PCAF from mouse, rat, and human origins and does not cross-react with GCN5, another HAT with similar functions. Recommended for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), PCAF (E-8) monoclonal antibody is an essential tool for researchers studying epigenetic regulation and the mechanisms of transcriptional control.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PCAF Antibody (E-8) References:
- PCAF fine-tunes hepatic metabolic syndrome, inflammatory disease, and cancer. | Wang, T., et al. 2018. J Cell Mol Med. 22: 5787-5800. PMID: 30216660
- Complex functions of Gcn5 and Pcaf in development and disease. | Koutelou, E., et al. 2021. Biochim Biophys Acta Gene Regul Mech. 1864: 194609. PMID: 32730897
- Upregulation of KAT2B and ESCO2 gene expression level in patients with rheumatoid arthritis. | Ghasemi, A., et al. 2023. Clin Rheumatol. 42: 253-259. PMID: 36104638
- Posttranslational regulation of the GCN5 and PCAF acetyltransferases. | Ononye, OE. and Downey, M. 2022. PLoS Genet. 18: e1010352. PMID: 36107838
- Nucleosome structural changes due to acetylation. | Bauer, WR., et al. 1994. J Mol Biol. 236: 685-90. PMID: 8114086
- A positive role for histone acetylation in transcription factor access to nucleosomal DNA. | Lee, DY., et al. 1993. Cell. 72: 73-84. PMID: 8422685
- Transcriptional silencing in yeast is associated with reduced nucleosome acetylation. | Braunstein, M., et al. 1993. Genes Dev. 7: 592-604. PMID: 8458576
- Tetrahymena histone acetyltransferase A: a homolog to yeast Gcn5p linking histone acetylation to gene activation. | Brownell, JE., et al. 1996. Cell. 84: 843-51. PMID: 8601308
- A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p. | Taunton, J., et al. 1996. Science. 272: 408-11. PMID: 8602529
- A p300/CBP-associated factor that competes with the adenoviral oncoprotein E1A. | Yang, XJ., et al. 1996. Nature. 382: 319-24. PMID: 8684459
- Transcriptional repression by YY1 is mediated by interaction with a mammalian homolog of the yeast global regulator RPD3. | Yang, WM., et al. 1996. Proc Natl Acad Sci U S A. 93: 12845-50. PMID: 8917507
- Identification of mouse histone deacetylase 1 as a growth factor-inducible gene. | Bartl, S., et al. 1997. Mol Cell Biol. 17: 5033-43. PMID: 9271381
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PCAF Antibody (E-8) | sc-13124 | 200 µg/ml | $322.00 | |||
PCAF Antibody (E-8): m-IgG Fc BP-HRP Bundle | sc-528263 | 200 µg Ab; 10 µg BP | $361.00 | |||
PCAF Antibody (E-8): m-IgGκ BP-HRP Bundle | sc-520596 | 200 µg Ab, 40 µg BP | $361.00 | |||
PCAF Antibody (E-8): m-IgG1 BP-HRP Bundle | sc-542846 | 200 µg Ab; 20 µg BP | $361.00 | |||
PCAF Antibody (E-8) AC | sc-13124 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PCAF Antibody (E-8) HRP | sc-13124 HRP | 200 µg/ml | $322.00 | |||
PCAF Antibody (E-8) FITC | sc-13124 FITC | 200 µg/ml | $336.00 | |||
PCAF Antibody (E-8) PE | sc-13124 PE | 200 µg/ml | $349.00 | |||
PCAF Antibody (E-8) Alexa Fluor® 488 | sc-13124 AF488 | 200 µg/ml | $364.00 | |||
PCAF Antibody (E-8) Alexa Fluor® 546 | sc-13124 AF546 | 200 µg/ml | $364.00 | |||
PCAF Antibody (E-8) Alexa Fluor® 594 | sc-13124 AF594 | 200 µg/ml | $364.00 | |||
PCAF Antibody (E-8) Alexa Fluor® 647 | sc-13124 AF647 | 200 µg/ml | $364.00 | |||
PCAF Antibody (E-8) Alexa Fluor® 680 | sc-13124 AF680 | 200 µg/ml | $364.00 | |||
PCAF Antibody (E-8) Alexa Fluor® 790 | sc-13124 AF790 | 200 µg/ml | $364.00 |