PBGS Antibody (A-7): sc-271585
- PBGS Antibody (A-7) is a mouse monoclonal IgG1 κ PBGS antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of PBGS of human origin
- PBGS Antibody (A-7) is recommended for detection of PBGS of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-PBGS Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PBGS Antibody (A-7) for WB and IHC(P) applications. These reagents are now offered in bundles with PBGS Antibody (A-7) (see ordering information below).
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PBGS Antibody (A-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects PBGS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PBGS (A-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PBGS protein, or porphobilinogen synthase, plays a crucial role in heme biosynthesis, essential for oxygen transport and electron transfer in various biological processes. PBGS enzyme catalyzes the condensation of two molecules of δ-aminolevulinate to form porphobilinogen, a key precursor in heme biosynthetic pathway. PBGS requires zinc as a cofactor for enzymatic activity, and lead inhibition can lead to severe health issues. Defects in ALAD gene can result in increased susceptibility to lead poisoning and acute hepatic porphyria, a group of inherited disorders characterized by partial enzyme defects in heme biosynthesis. Two common alleles, ALAD 2 and ALAD 1, influence individual responses to environmental lead exposure, with ALAD 2 carriers exhibiting significantly higher blood lead levels compared to ALAD 1 homozygotes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PBGS Antibody (A-7) References:
- Hematological changes in rats chronically exposed to oral aluminum. | Farina, M., et al. 2005. Toxicology. 209: 29-37. PMID: 15725511
- The activation mechanism of human porphobilinogen synthase by 2-mercaptoethanol: intrasubunit transfer of a reserve zinc ion and coordination with three cysteines in the active center. | Sawada, N., et al. 2005. J Biol Inorg Chem. 10: 199-207. PMID: 15747133
- 2,3-Dimercapto-1-propanol does not alter the porphobilinogen synthase inhibition but decreases the mercury content in liver and kidney of suckling rats exposed to HgCl2. | Roza, T., et al. 2005. Basic Clin Pharmacol Toxicol. 96: 302-8. PMID: 15755313
- Comparative study on two freshwater invertebrates for monitoring environmental lead exposure. | Aisemberg, J., et al. 2005. Toxicology. 210: 45-53. PMID: 15804457
- Du-zhong (Eucommia ulmoides Oliv.) cortex water extract alters heme biosynthesis and erythrocyte antioxidant defense system in lead-administered rats. | Lee, MK., et al. 2005. J Med Food. 8: 86-92. PMID: 15857215
- Changes in erythrocyte enzymes in humans long-term exposed to pesticides: influence of several markers of individual susceptibility. | Hernández, AF., et al. 2005. Toxicol Lett. 159: 13-21. PMID: 15922524
- Human erythrocyte delta-aminolevulinate dehydratase inhibition by monosaccharides is not mediated by oxidation of enzyme sulfhydryl groups. | Gabriel, D., et al. 2005. Cell Biol Int. 29: 669-74. PMID: 15950499
- Morpheeins--a new structural paradigm for allosteric regulation. | Jaffe, EK. 2005. Trends Biochem Sci. 30: 490-7. PMID: 16023348
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PBGS Antibody (A-7) | sc-271585 | 200 µg/ml | $316.00 | |||
PBGS Antibody (A-7): m-IgG Fc BP-HRP Bundle | sc-529215 | 200 µg Ab; 10 µg BP | $354.00 | |||
PBGS Antibody (A-7): m-IgGκ BP-HRP Bundle | sc-521988 | 200 µg Ab, 40 µg BP | $354.00 | |||
PBGS Antibody (A-7): m-IgG1 BP-HRP Bundle | sc-543392 | 200 µg Ab; 20 µg BP | $354.00 | |||
PBGS Antibody (A-7) AC | sc-271585 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PBGS Antibody (A-7) HRP | sc-271585 HRP | 200 µg/ml | $316.00 | |||
PBGS Antibody (A-7) FITC | sc-271585 FITC | 200 µg/ml | $330.00 | |||
PBGS Antibody (A-7) PE | sc-271585 PE | 200 µg/ml | $343.00 | |||
PBGS Antibody (A-7) Alexa Fluor® 488 | sc-271585 AF488 | 200 µg/ml | $357.00 | |||
PBGS Antibody (A-7) Alexa Fluor® 546 | sc-271585 AF546 | 200 µg/ml | $357.00 | |||
PBGS Antibody (A-7) Alexa Fluor® 594 | sc-271585 AF594 | 200 µg/ml | $357.00 | |||
PBGS Antibody (A-7) Alexa Fluor® 647 | sc-271585 AF647 | 200 µg/ml | $357.00 | |||
PBGS Antibody (A-7) Alexa Fluor® 680 | sc-271585 AF680 | 200 µg/ml | $357.00 | |||
PBGS Antibody (A-7) Alexa Fluor® 790 | sc-271585 AF790 | 200 µg/ml | $357.00 |