PBEF Antibody (F-8): sc-393510

PBEF Antibody (F-8) is a mouse monoclonal IgG1 antibody that detects pre-B cell-enhancing factor (PBEF) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PBEF, also known as nicotinamide phosphoribosyltransferase (Nampt) or visfatin, plays a crucial role in NAD biosynthesis, catalyzing the conversion of nicotinamide and 5-phosphoribosyl-1-pyrophosphate into nicotinamide mononucleotide, a key precursor in NAD production. NAD is essential for various cellular functions, including energy metabolism and DNA repair, making PBEF a vital player in maintaining cellular health and function. PBEF is highly enriched in visceral fat and plasma expression levels increase during obesity, linking PBEF to metabolic disorders. PBEF is primarily located in the cytoplasm and predominantly expressed in bone marrow, muscle, and liver tissues, but can also be found in placenta, lung, kidney, and heart, highlighting diverse biological roles across different tissues. Anti-PBEF antibody (F-8) serves as an invaluable tool for researchers investigating PBEF implications in metabolic regulation and related diseases.

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PBEF Antibody (F-8) References:

1. Gene expression profiling of peripheral blood mononuclear leukocytes from psoriasis patients identifies new immune regulatory molecules.  |  Koczan, D., et al. 2005. Eur J Dermatol. 15: 251-7. PMID: 16048752
2. Correlation of circulating full-length visfatin (PBEF/NAMPT) with metabolic parameters in subjects with and without diabetes: a cross-sectional study.  |  Retnakaran, R., et al. 2008. Clin Endocrinol (Oxf). 69: 885-93. PMID: 18410550
3. Relationship between cerebrospinal fluid visfatin (PBEF/Nampt) levels and adiposity in humans.  |  Hallschmid, M., et al. 2009. Diabetes. 58: 637-40. PMID: 19095760
4. Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is constitutively released from human hepatocytes.  |  Garten, A., et al. 2010. Biochem Biophys Res Commun. 391: 376-81. PMID: 19912992
5. Association of increased visfatin/PBEF/NAMPT circulating concentrations and gene expression levels in peripheral blood cells with lipid metabolism and fatty liver in human morbid obesity.  |  Catalán, V., et al. 2011. Nutr Metab Cardiovasc Dis. 21: 245-53. PMID: 20106640
6. Circulating and local visfatin/Nampt/PBEF levels in spontaneously hypertensive rats, stroke-prone spontaneously hypertensive rats and Wistar-Kyoto rats.  |  Wang, P., et al. 2010. J Physiol Sci. 60: 317-24. PMID: 20658215
7. Visfatin/PBEF/Nampt induces EMMPRIN and MMP-9 production in macrophages via the NAMPT-MAPK (p38, ERK1/2)-NF-κB signaling pathway.  |  Fan, Y., et al. 2011. Int J Mol Med. 27: 607-15. PMID: 21327328
8. Nampt/visfatin/PBEF affects expression of myogenic regulatory factors and is regulated by interleukin-6 in chicken skeletal muscle cells.  |  Krzysik-Walker, SM., et al. 2011. Comp Biochem Physiol A Mol Integr Physiol. 159: 413-21. PMID: 21545843
9. Epigenetic characterization of the PBEF and TIMP-2 genes in the developing placentae of normal mice.  |  Kim, HR., et al. 2011. BMB Rep. 44: 535-40. PMID: 21871178
10. Rapid and sensitive detection of Nampt (PBEF/visfatin) in human serum using an ssDNA aptamer-based capacitive biosensor.  |  Park, JW., et al. 2012. Biosens Bioelectron. 38: 233-8. PMID: 22704839

The preferred PBEF antibody is PBEF Antibody (E-3): sc-393444.