paxillin Antibody (B-2): sc-365379

paxillin Antibody (B-2) is a mouse monoclonal IgG2b kappa light chain antibody specific for an epitope mapping between amino acids 17-47 near the N-terminus of human paxillin. Paxillin, also known as PXN, is a focal adhesion phosphoprotein localized to the cytoskeleton, playing a pivotal role in cell adhesion, migration, and signal transduction pathways. Paxillin interacts with a variety of proteins, including focal adhesion kinase (FAK), Src family kinases, vinculin, and integrins, serving as a scaffold that assembles multiprotein complexes at focal adhesion sites. These interactions are crucial for transmitting signals from the extracellular matrix to the intracellular environment, influencing processes like cytoskeletal reorganization and cell motility. Phosphorylation of paxillin occurs in response to stimuli such as platelet-derived growth factor (PDGF) treatment and v-src transformation, with kinases like FAK and PYK2 phosphorylating specific tyrosine residues, notably Tyr118. During mitosis, paxillin levels decrease due to proteolytic downregulation, and paxillin undergoes alternative phosphorylation on serine residues, highlighting a role in cell cycle regulation. anti-paxillin antibody (B-2) detects the α, β, and γ isoforms of paxillin from mouse, rat, human, equine, and canine origins in applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, paxillin (B-2) antibody is a valuable tool for researchers studying the interactions and functions of paxillin in cellular signaling pathways across different species.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

paxillin Antibody (B-2) References:

1. Complement receptor 3 (CR3, Mac-1, integrin alpha M beta 2, CD11b/CD18) is required for tyrosine phosphorylation of paxillin in adherent and nonadherent neutrophils.  |  Graham, IL., et al. 1994. J Cell Biol. 127: 1139-47. PMID: 7525604
2. Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL.  |  Salgia, R., et al. 1995. J Biol Chem. 270: 5039-47. PMID: 7534286
3. Characterization of tyrosine phosphorylation of paxillin in vitro by focal adhesion kinase.  |  Bellis, SL., et al. 1995. J Biol Chem. 270: 17437-41. PMID: 7615549
4. Tyrosine phosphorylation and enhanced expression of paxillin during neuronal differentiation in vitro.  |  Leventhal, PS. and Feldman, EL. 1996. J Biol Chem. 271: 5957-60. PMID: 8626373
5. Identification of LIM3 as the principal determinant of paxillin focal adhesion localization and characterization of a novel motif on paxillin directing vinculin and focal adhesion kinase binding.  |  Brown, MC., et al. 1996. J Cell Biol. 135: 1109-23. PMID: 8922390
6. Paxillin is tyrosine-phosphorylated by and preferentially associates with the calcium-dependent tyrosine kinase in rat liver epithelial cells.  |  Li, X. and Earp, HS. 1997. J Biol Chem. 272: 14341-8. PMID: 9162070
7. Mitosis specific serine phosphorylation and downregulation of one of the focal adhesion protein, paxillin.  |  Yamaguchi, R., et al. 1997. Oncogene. 15: 1753-61. PMID: 9362441

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-paxillin Antibody (B-2) has 31 citations in a variety of scientific publications.

Primary antibodies like Anti-paxillin Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

paxillin Antibody (B-2) Specifications

Alternate Product Name: paxillin

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 17-47

Epitope Target: paxillin

Epitope Species: human

Product Reactivity/Detection: paxillin isoforms α, β and γ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 31 citations in various scientific publications

Available Conjugates/Forms for paxillin Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin