patched/PTCH1 Antibody (H-10): sc-518102
- patched/PTCH1 Antibody (H-10) is a mouse monoclonal IgG1 κ patched/PTCH1 antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 270-296 within an extracellular domain of patched of human origin
- recommended for detection of patched of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-patched/PTCH1 Antibody (H-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for patched/PTCH1 Antibody (H-10) for WB applications. These reagents are now offered in bundles with patched/PTCH1 Antibody (H-10) (see ordering information below).
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Patched Antibody (H-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects patched in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Patched (H-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Patched plays a crucial role in the hedgehog signaling pathway, which regulates cell growth and differentiation during embryonic development. Patched functions as a receptor that inhibits hedgehog proteins′ signaling activity, maintaining a balance in cellular signaling vital for proper tissue patterning and organogenesis. Mutations in patched can lead to uncontrolled cell proliferation and are associated with various cancers, including basal cell carcinoma, which affects many individuals annually in the United States. Patched has been shown to interact with other key proteins in the pathway, such as Sonic hedgehog and GLI transcription factors, highlighting patched′s importance in cellular communication and developmental processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
patched/PTCH1 Antibody (H-10) References:
- Desert Hedgehog/Patched 1 signaling specifies fetal Leydig cell fate in testis organogenesis. | Yao, HH., et al. 2002. Genes Dev. 16: 1433-40. PMID: 12050120
- The patched polymorphism Pro1315Leu (C3944T) may modulate the association between use of oral contraceptives and breast cancer risk. | Chang-Claude, J., et al. 2003. Int J Cancer. 103: 779-83. PMID: 12516098
- Repression of smoothened by patched-dependent (pro-)vitamin D3 secretion. | Bijlsma, MF., et al. 2006. PLoS Biol. 4: e232. PMID: 16895439
- Multipotent CD15+ cancer stem cells in patched-1-deficient mouse medulloblastoma. | Ward, RJ., et al. 2009. Cancer Res. 69: 4682-90. PMID: 19487286
- T cell development critically depends on prethymic stromal patched expression. | Uhmann, A., et al. 2011. J Immunol. 186: 3383-91. PMID: 21317383
- Inhibition of patched-1 prevents injury-induced neointimal hyperplasia. | Redmond, EM., et al. 2013. Arterioscler Thromb Vasc Biol. 33: 1960-4. PMID: 23766265
- Natural paniceins from mediterranean sponge inhibit the multidrug resistance activity of Patched and increase chemotherapy efficiency on melanoma cells. | Fiorini, L., et al. 2015. Oncotarget. 6: 22282-97. PMID: 26068979
- The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog. | Stone, DM., et al. 1996. Nature. 384: 129-34. PMID: 8906787
- Hedgehog and patched gene expression in adult ocular tissues. | Takabatake, T., et al. 1997. FEBS Lett. 410: 485-9. PMID: 9237688
- Ptch2, a second mouse Patched gene is co-expressed with Sonic hedgehog. | Motoyama, J., et al. 1998. Nat Genet. 18: 104-6. PMID: 9462734
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
patched/PTCH1 Antibody (H-10) | sc-518102 | 200 µg/ml | $322.00 | |||
patched/PTCH1 Antibody (H-10): m-IgG Fc BP-HRP Bundle | sc-531615 | 200 µg Ab; 10 µg BP | $361.00 | |||
patched/PTCH1 Antibody (H-10): m-IgGκ BP-HRP Bundle | sc-525369 | 200 µg Ab, 40 µg BP | $361.00 | |||
patched/PTCH1 Antibody (H-10): m-IgG1 BP-HRP Bundle | sc-544657 | 200 µg Ab; 20 µg BP | $361.00 | |||
patched/PTCH1 Antibody (H-10) AC | sc-518102 AC | 500 µg/ml, 25% agarose | $424.00 | |||
patched/PTCH1 Antibody (H-10) HRP | sc-518102 HRP | 200 µg/ml | $322.00 | |||
patched/PTCH1 Antibody (H-10) FITC | sc-518102 FITC | 200 µg/ml | $336.00 | |||
patched/PTCH1 Antibody (H-10) PE | sc-518102 PE | 200 µg/ml | $349.00 | |||
patched/PTCH1 Antibody (H-10) Alexa Fluor® 488 | sc-518102 AF488 | 200 µg/ml | $364.00 | |||
patched/PTCH1 Antibody (H-10) Alexa Fluor® 546 | sc-518102 AF546 | 200 µg/ml | $364.00 | |||
patched/PTCH1 Antibody (H-10) Alexa Fluor® 594 | sc-518102 AF594 | 200 µg/ml | $364.00 | |||
patched/PTCH1 Antibody (H-10) Alexa Fluor® 647 | sc-518102 AF647 | 200 µg/ml | $364.00 | |||
patched/PTCH1 Antibody (H-10) Alexa Fluor® 680 | sc-518102 AF680 | 200 µg/ml | $364.00 | |||
patched/PTCH1 Antibody (H-10) Alexa Fluor® 790 | sc-518102 AF790 | 200 µg/ml | $364.00 |