patched/PTCH1 Antibody (A-2): sc-518044

Patched Antibody (A-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects patched protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-patched antibody (A-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Patched protein plays a crucial role in the hedgehog signaling pathway, which regulates cell growth and differentiation during embryonic development. Patched functions as a receptor that inhibits hedgehog protein activity, controlling the expression of downstream targets, including the Wnt family of proteins. This regulation is vital because aberrations in hedgehog signaling, often due to mutations in the patched gene, can lead to uncontrolled cell proliferation and are implicated in various cancers, including basal cell carcinoma, affecting approximately 750,000 individuals annually in the United States. The interaction between patched and Sonic hedgehog highlights the complexity of this signaling pathway, as patched 2, another family member, has been found to be coexpressed with Sonic hedgehog, suggesting a nuanced regulatory mechanism that could be targeted for therapeutic interventions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-patched/PTCH1 Antibody (A-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-patched/PTCH1 Antibody (A-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

patched/PTCH1 Antibody (A-2) Specifications

Alternate Product Name: PTCH1

Product Clone Name: A-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1401-1429

Epitope Target: patched

Epitope Species: human

Product Reactivity/Detection: patched

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for patched/PTCH1 Antibody (A-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred patched/PTCH1 antibody is patched/PTCH1 Antibody (H-10): sc-518102.