PARP-2 Antibody (F-3): sc-393310

PARP-2 Antibody (F-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects PARP-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PARP-2 Antibody (F-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Poly(ADP-ribose) polymerase-2 (PARP-2) plays a crucial role in the base excision repair (BER) pathway, where PARP-2 catalyzes the poly(ADP-ribosyl)ation of nuclear proteins, a vital post-translational modification that occurs in response to DNA damage. This modification is essential for the recruitment of DNA repair proteins to sites of damage, thereby facilitating the repair of DNA strand breaks and maintaining genomic stability. PARP-2 is predominantly located in the nucleus and is particularly abundant in actively dividing tissues such as the kidney, skeletal muscle, liver, heart, and spleen, highlighting PARP-2′s importance in cellular proliferation and repair mechanisms. Additionally, human PARP-2 is mapped to chromosome 14q11.2-q12, underscoring PARP-2′s genetic significance in various biological processes.

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PARP-2 Antibody (F-3) References:

1. PARP-2, A novel mammalian DNA damage-dependent poly(ADP-ribose) polymerase.  |  Amé, JC., et al. 1999. J Biol Chem. 274: 17860-8. PMID: 10364231
2. Poly(ADP-ribose) polymerase-2 (PARP-2) is required for efficient base excision DNA repair in association with PARP-1 and XRCC1.  |  Schreiber, V., et al. 2002. J Biol Chem. 277: 23028-36. PMID: 11948190
3. Functional interaction between PARP-1 and PARP-2 in chromosome stability and embryonic development in mouse.  |  Ménissier de Murcia, J., et al. 2003. EMBO J. 22: 2255-63. PMID: 12727891
4. PARP-1 and PARP-2 interact with nucleophosmin/B23 and accumulate in transcriptionally active nucleoli.  |  Meder, VS., et al. 2005. J Cell Sci. 118: 211-22. PMID: 15615785
5. Discovery of quinazolinone and quinoxaline derivatives as potent and selective poly(ADP-ribose) polymerase-1/2 inhibitors.  |  Iwashita, A., et al. 2005. FEBS Lett. 579: 1389-93. PMID: 15733846
6. PARP inhibitors for cancer therapy.  |  Curtin, NJ. 2005. Expert Rev Mol Med. 7: 1-20. PMID: 15836799
7. PARP-2 sustains erythropoiesis in mice by limiting replicative stress in erythroid progenitors.  |  Farrés, J., et al. 2015. Cell Death Differ. 22: 1144-57. PMID: 25501596
8. PARP-1/PARP-2 double deficiency in mouse T cells results in faulty immune responses and T lymphomas.  |  Navarro, J., et al. 2017. Sci Rep. 7: 41962. PMID: 28181505
9. Distinct roles for PARP-1 and PARP-2 in c-Myc-driven B-cell lymphoma in mice.  |  Galindo-Campos, MA., et al. 2022. Blood. 139: 228-239. PMID: 34359075
10. Deficiency of PARP-1 and PARP-2 in the mouse uterus results in decidualization failure and pregnancy loss.  |  Kelleher, AM., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34580230
11. Structure-based design, synthesis, and evaluation of inhibitors with high selectivity for PARP-1 over PARP-2.  |  Yu, J., et al. 2022. Eur J Med Chem. 227: 113898. PMID: 34656898
12. PARP-2 mediates cardiomyocyte aging and damage induced by doxorubicin through SIRT1 Inhibition.  |  Huang, C., et al. 2024. Apoptosis. 29: 816-834. PMID: 38281279

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PARP-2 Antibody (F-3) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-PARP-2 Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PARP-2 Antibody (F-3) Specifications

Alternate Product Name: PARP-2

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 103-138

Epitope Target: PARP-2

Epitope Species: human

Product Reactivity/Detection: PARP-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for PARP-2 Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred PARP-2 antibody is PARP-2 Antibody (F-8): sc-393343.