PARL Antibody (F-3): sc-514836

PARL Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects PARL protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PARL (F-3) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The presenilins associated rhombiod-like protein (PARL) is a crucial mitochondrial intramembrane-cleaving protease that belongs to the S54 family of proteins, playing a significant role in intramembrane regulated proteolysis. PARL is essential for cleaving signaling proteins at intracellular membranes, which releases active fragments vital for signal transduction cascades. PARL utilizes a triad of histidine, serine, and asparagine residues to effectively cleave type-1 transmembrane domains, thereby influencing various cellular processes. PARL is a multi-pass membrane protein that localizes to both inner and outer mitochondrial membranes, but can also be detected in the nucleus following proteolytic processing of P-beta. PARL co-localizes with presenilins PSEN1 and PSEN2, which are implicated in familial Alzheimer′s disease, highlighting its importance in neurodegenerative conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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PARL Antibody (F-3) References:

1. PAMP and PARL, two novel putative metalloproteases interacting with the COOH-terminus of Presenilin-1 and -2.  |  Pellegrini, L., et al. 2001. J Alzheimers Dis. 3: 181-190. PMID: 12214059
2. The rhomboids: a nearly ubiquitous family of intramembrane serine proteases that probably evolved by multiple ancient horizontal gene transfers.  |  Koonin, EV., et al. 2003. Genome Biol. 4: R19. PMID: 12620104
3. Self-regulated cleavage of the mitochondrial intramembrane-cleaving protease PARL yields Pbeta, a nuclear-targeted peptide.  |  Sík, A., et al. 2004. J Biol Chem. 279: 15323-9. PMID: 14732705
4. The mitochondrial rhomboid protease PSARL is a new candidate gene for type 2 diabetes.  |  Walder, K., et al. 2005. Diabetologia. 48: 459-68. PMID: 15729572
5. A split-brain model of Alzheimer's disease? Behavioral evidence for comparable intra and interhemispheric decline.  |  Reuter-Lorenz, PA. and Mikels, JA. 2005. Neuropsychologia. 43: 1307-17. PMID: 15949515
6. OPA1 and PARL keep a lid on apoptosis.  |  Gottlieb, E. 2006. Cell. 126: 27-9. PMID: 16839872
7. Mitochondrial rhomboid PARL regulates cytochrome c release during apoptosis via OPA1-dependent cristae remodeling.  |  Cipolat, S., et al. 2006. Cell. 126: 163-75. PMID: 16839884
8. Genome-wide Survival Study Identifies PARL as a Novel Locus for Clinical Progression and Neurodegeneration in Alzheimer's Disease.  |  Chen, SD., et al. 2023. Biol Psychiatry. 94: 732-742. PMID: 36870520
9. STOML2 restricts mitophagy and increases chemosensitivity in pancreatic cancer through stabilizing PARL-induced PINK1 degradation.  |  Qin, C., et al. 2023. Cell Death Dis. 14: 191. PMID: 36906621
10. Mitochondrial defects caused by PARL deficiency lead to arrested spermatogenesis and ferroptosis.  |  Radaelli, E., et al. 2023. Elife. 12: PMID: 37505079
11. Disruption of Mitophagy Flux through the PARL-PINK1 Pathway by CHCHD10 Mutations or CHCHD10 Depletion.  |  Liu, T., et al. 2023. Cells. 12: PMID: 38132101
12. NPR1 promotes cisplatin resistance by inhibiting PARL-mediated mitophagy-dependent ferroptosis in gastric cancer.  |  Wu, C., et al. 2024. Cell Biol Toxicol. 40: 93. PMID: 39476297

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PARL Antibody (F-3) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-PARL Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PARL Antibody (F-3) Specifications

Alternate Product Name: PARL

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 80-371 deletion 204-253

Epitope Target: PARL

Epitope Species: human

Product Reactivity/Detection: PARL

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for PARL Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin