Parkin Antibody (H-8): sc-136989
- Parkin Antibody (H-8) is a mouse monoclonal IgG2a κ, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 61-360 mapping within an internal region of Parkin of human origin
- Parkin Antibody (H-8) is recommended for detection of Parkin of human origin by WB, IP, IF and ELISA
- Anti-Parkin Antibody (H-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Parkin Antibody (H-8). This work is in progress.
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SEE ALSO...
Parkin Antibody (H-8) is a mouse monoclonal IgG2a kappa light chain antibody targeting human Parkin, also referred to as PRKN or parkin RBR E3 ubiquitin protein ligase, and is validated for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Parkin is a 465-amino acid E3 ubiquitin ligase encoded by the PARK2 gene, which plays a pivotal role in tagging proteins for degradation, a process essential for maintaining cellular homeostasis. Structurally, Parkin contains an N-terminal ubiquitin-like domain and several zinc-finger motifs, including RING (Really Interesting New Gene) domains, which are critical for its E3 ligase activity. These domains facilitate interactions with substrate proteins and the ubiquitin-conjugating machinery, enabling Parkin to regulate protein turnover effectively. The integrity of these structural motifs is vital for Parkin′s function, as mutations within these regions often disrupt its activity and are linked to autosomal recessive juvenile Parkinson′s disease. anti-Parkin antibody (H-8) was generated against amino acids 61-360 of human Parkin, mapping within an internal region of the protein. Parkin is predominantly cytoplasmic but translocates to the mitochondria in response to cellular stress, initiating mitophagy to remove damaged mitochondria. Parkin monoclonal antibody (H-8) is a valuable reagent for studying Parkin′s structural features and their implications in cellular pathways and neurodegenerative diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Parkin Antibody (H-8) | sc-136989 | 200 µg/ml | $322.00 | |||
Parkin Antibody (H-8) AC | sc-136989 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Parkin Antibody (H-8) HRP | sc-136989 HRP | 200 µg/ml | $322.00 | |||
Parkin Antibody (H-8) FITC | sc-136989 FITC | 200 µg/ml | $336.00 | |||
Parkin Antibody (H-8) PE | sc-136989 PE | 200 µg/ml | $349.00 | |||
Parkin Antibody (H-8) Alexa Fluor® 488 | sc-136989 AF488 | 200 µg/ml | $364.00 | |||
Parkin Antibody (H-8) Alexa Fluor® 546 | sc-136989 AF546 | 200 µg/ml | $364.00 | |||
Parkin Antibody (H-8) Alexa Fluor® 594 | sc-136989 AF594 | 200 µg/ml | $364.00 | |||
Parkin Antibody (H-8) Alexa Fluor® 647 | sc-136989 AF647 | 200 µg/ml | $364.00 | |||
Parkin Antibody (H-8) Alexa Fluor® 680 | sc-136989 AF680 | 200 µg/ml | $364.00 | |||
Parkin Antibody (H-8) Alexa Fluor® 790 | sc-136989 AF790 | 200 µg/ml | $364.00 |