PARD6B Antibody (B-10): sc-166405

PARD6B Antibody (B-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects PARD6B of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PARD6B (B-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PARD6B, a 345 amino acid polypeptide, plays a crucial role in cellular asymmetry, which is essential for the proper development of multicellular organisms. PARD6B contains a PDZ domain and a CRIB-like motif that facilitates interactions with GTP-bound Rac and Cdc42, as well as atypical protein kinase C isoforms PKCι/λ and PKCΩ. These interactions are vital for the formation of a ternary complex that is implicated in establishing normal tight junctions at epithelial cell-cell contacts and in the polarization of mother cells prior to asymmetric cell division in organisms such as C. elegans. PARD6B is involved in localizing or maintaining PARD3 at the cell periphery, thereby influencing cell polarity and growth. PARD6B is predominantly expressed in the pancreas and both adult and fetal kidneys, with weaker expression observed in the placenta and lung, highlighting its significance in various physiological processes.

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PARD6B Antibody (B-10) References:

1. A human homolog of the C. elegans polarity determinant Par-6 links Rac and Cdc42 to PKCzeta signaling and cell transformation.  |  Qiu, RG., et al. 2000. Curr Biol. 10: 697-707. PMID: 10873802
2. The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42.  |  Joberty, G., et al. 2000. Nat Cell Biol. 2: 531-9. PMID: 10934474
3. A mammalian PAR-3-PAR-6 complex implicated in Cdc42/Rac1 and aPKC signalling and cell polarity.  |  Lin, D., et al. 2000. Nat Cell Biol. 2: 540-7. PMID: 10934475
4. Cell polarity: new PARtners for Cdc42 and Rac.  |  Kim, SK. 2000. Nat Cell Biol. 2: E143-5. PMID: 10934484
5. The mammalian homologue of the Caenorhabditis elegans polarity protein PAR-6 is a binding partner for the Rho GTPases Cdc42 and Rac1.  |  Johansson, A., et al. 2000. J Cell Sci. 113 (Pt 18): 3267-75. PMID: 10954424
6. Cell polarity: Scaffold proteins par excellence.  |  Brazil, DP. and Hemmings, BA. 2000. Curr Biol. 10: R592-4. PMID: 10985375
7. Human homologues of the Caenorhabditis elegans cell polarity protein PAR6 as an adaptor that links the small GTPases Rac and Cdc42 to atypical protein kinase C.  |  Noda, Y., et al. 2001. Genes Cells. 6: 107-19. PMID: 11260256
8. par-6, a gene involved in the establishment of asymmetry in early C. elegans embryos, mediates the asymmetric localization of PAR-3.  |  Watts, JL., et al. 1996. Development. 122: 3133-40. PMID: 8898226

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PARD6B Antibody (B-10) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-PARD6B Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PARD6B Antibody (B-10) Specifications

Alternate Product Name: PARD6B

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 308-371

Epitope Target: PARD6B

Epitope Species: mouse

Product Reactivity/Detection: PARD6B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 23 citations in various scientific publications

Available Conjugates/Forms for PARD6B Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin