PARD6A Antibody (C-3): sc-365323

PARD6A Antibody (C-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects PARD6A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PARD6A (C-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PARD6A protein, also known as PAR6C, plays a crucial role in establishing cellular asymmetry, which is vital for proper development of multicellular organisms. This 345 amino acid polypeptide features a PDZ domain and a CRIB-like motif that facilitates interaction with GTP-bound Rac and Cdc42, as well as atypical protein kinase C isoforms PKCι/λ and PKCΩ. These interactions are essential for forming a ternary complex that contributes to establishing tight junctions at epithelial cell-cell contacts and is involved in polarizing mother cells prior to asymmetric cell division in organisms such as C. elegans. PARD6A is predominantly expressed in pancreas, skeletal muscle, brain, and heart, with weaker expression observed in kidney and placenta. Additionally, PARD6A interacts with PARD3, helping to localize or maintain PARD3 at cell periphery, further underscoring its importance in cellular organization and signaling pathways.

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PARD6A Antibody (C-3) References:

1. A human homolog of the C. elegans polarity determinant Par-6 links Rac and Cdc42 to PKCzeta signaling and cell transformation.  |  Qiu, RG., et al. 2000. Curr Biol. 10: 697-707. PMID: 10873802
2. The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42.  |  Joberty, G., et al. 2000. Nat Cell Biol. 2: 531-9. PMID: 10934474
3. A mammalian PAR-3-PAR-6 complex implicated in Cdc42/Rac1 and aPKC signalling and cell polarity.  |  Lin, D., et al. 2000. Nat Cell Biol. 2: 540-7. PMID: 10934475
4. Cell polarity: new PARtners for Cdc42 and Rac.  |  Kim, SK. 2000. Nat Cell Biol. 2: E143-5. PMID: 10934484
5. The mammalian homologue of the Caenorhabditis elegans polarity protein PAR-6 is a binding partner for the Rho GTPases Cdc42 and Rac1.  |  Johansson, A., et al. 2000. J Cell Sci. 113 (Pt 18): 3267-75. PMID: 10954424
6. Cell polarity: Scaffold proteins par excellence.  |  Brazil, DP. and Hemmings, BA. 2000. Curr Biol. 10: R592-4. PMID: 10985375
7. Human homologues of the Caenorhabditis elegans cell polarity protein PAR6 as an adaptor that links the small GTPases Rac and Cdc42 to atypical protein kinase C.  |  Noda, Y., et al. 2001. Genes Cells. 6: 107-19. PMID: 11260256
8. Identification and characterization of human Inscuteable gene in silico.  |  Katoh, M. and Katoh, M. 2003. Int J Mol Med. 11: 111-6. PMID: 12469229
9. A genome-wide screen of CREB occupancy identifies the RhoA inhibitors Par6C and Rnd3 as regulators of BDNF-induced synaptogenesis.  |  Lesiak, A., et al. 2013. PLoS One. 8: e64658. PMID: 23762244
10. A Radiosensitivity Prediction Model Developed Based on Weighted Correlation Network Analysis of Hypoxia Genes for Lower-Grade Glioma.  |  Du, Z., et al. 2022. Front Oncol. 12: 757686. PMID: 35280808
11. PARD6A promotes lung adenocarcinoma cell proliferation and invasion through Serpina3.  |  Hu, L., et al. 2024. Cancer Gene Ther. 31: 1696-1707. PMID: 39300216
12. par-6, a gene involved in the establishment of asymmetry in early C. elegans embryos, mediates the asymmetric localization of PAR-3.  |  Watts, JL., et al. 1996. Development. 122: 3133-40. PMID: 8898226

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PARD6A Antibody (C-3) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-PARD6A Antibody (C-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PARD6A Antibody (C-3) Specifications

Alternate Product Name: PARD6A

Product Clone Name: C-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2-27

Epitope Target: PARD6A

Epitope Species: human

Product Reactivity/Detection: PARD6A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for PARD6A Antibody (C-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin