Parafibromin Antibody (G-9): sc-365749
- Parafibromin Antibody (G-9) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 97-131 within an internal region of Parafibromin of human origin
- recommended for detection of Parafibromin of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and porcine
- See Parafibromin (2H1): sc-33638 for Parafibromin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Parafibromin Antibody (G-9) for WB applications. These reagents are now offered in bundles with Parafibromin Antibody (G-9) (see ordering information below).
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Parafibromin Antibody (G-9) is a mouse monoclonal IgG1 light chain antibody that detects Parafibromin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Parafibromin plays a critical role as a tumor suppressor, encoded by the CDC73 gene, which is essential for regulating cell division and maintaining genomic stability. Parafibromin is located in the nucleus, where anti-Parafibromin antibody (G-9) identifies a key component of the RNA polymerase II/Paf1 complex, facilitating transcriptional regulation of genes through histone modification. Proper functioning of Parafibromin is vital, as dysregulation can lead to parathyroid tumors and other endocrine disorders. Parafibromin interacts with various proteins involved in transcriptional regulation, including the RNA polymerase II large subunit, which underscores its importance in cellular processes. Parafibromin monoclonal antibody (G-9) serves as an invaluable tool for researchers studying parathyroid carcinoma and related conditions, providing insights into molecular mechanisms underlying these diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Parafibromin Antibody (G-9) References:
- The HRPT2 tumor suppressor gene product parafibromin associates with human PAF1 and RNA polymerase II. | Yart, A., et al. 2005. Mol Cell Biol. 25: 5052-60. PMID: 15923622
- Parafibromin inhibits cancer cell growth and causes G1 phase arrest. | Zhang, C., et al. 2006. Biochem Biophys Res Commun. 350: 17-24. PMID: 16989776
- Immunohistochemical assessment of parafibromin in mouse and human tissues. | Porzionato, A., et al. 2006. J Anat. 209: 817-27. PMID: 17118068
- Nuclear localization of the parafibromin tumor suppressor protein implicated in the hyperparathyroidism-jaw tumor syndrome enhances its proapoptotic function. | Lin, L., et al. 2007. Mol Cancer Res. 5: 183-93. PMID: 17314275
- Downregulated parafibromin expression is a promising marker for pathogenesis, invasion, metastasis and prognosis of gastric carcinomas. | Zheng, HC., et al. 2008. Virchows Arch. 452: 147-55. PMID: 18080135
- Accuracy of combined protein gene product 9.5 and parafibromin markers for immunohistochemical diagnosis of parathyroid carcinoma. | Howell, VM., et al. 2009. J Clin Endocrinol Metab. 94: 434-41. PMID: 19017757
- The tumor suppressor parafibromin is required for posttranscriptional processing of histone mRNA. | Farber, LJ., et al. 2010. Mol Carcinog. 49: 215-23. PMID: 19908240
- Characterization of a new CDC73 missense mutation that impairs Parafibromin expression and nucleolar localization. | Masi, G., et al. 2014. PLoS One. 9: e97994. PMID: 24842573
- Cytoplasmic parafibromin/hCdc73 targets and destabilizes p53 mRNA to control p53-mediated apoptosis. | Jo, JH., et al. 2014. Nat Commun. 5: 5433. PMID: 25388829
- The in vitro and vivo effects of nuclear and cytosolic parafibromin expression on the aggressive phenotypes of colorectal cancer cells: a search of potential gene therapy target. | Zheng, HC., et al. 2017. Oncotarget. 8: 23603-23612. PMID: 28223542
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Parafibromin Antibody (G-9) | sc-365749 | 200 µg/ml | $322.00 | |||
Parafibromin Antibody (G-9): m-IgG Fc BP-HRP Bundle | sc-537830 | 200 µg Ab; 10 µg BP | $361.00 | |||
Parafibromin Antibody (G-9): m-IgGκ BP-HRP Bundle | sc-535251 | 200 µg Ab; 40 µg BP | $361.00 | |||
Parafibromin Antibody (G-9): m-IgG1 BP-HRP Bundle | sc-545385 | 200 µg Ab; 20 µg BP | $361.00 | |||
Parafibromin (G-9) Neutralizing Peptide | sc-365749 P | 100 µg/0.5 ml | $69.00 |