Parafibromin Antibody (2H1): sc-33638

Parafibromin Antibody (2H1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Parafibromin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Parafibromin antibody (2H1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Parafibromin, encoded by the CDC73 gene, plays a critical role in regulating gene expression and is a key component of the RNA polymerase II/Paf1 complex, which is essential for histone modification and transcriptional regulation. This complex is vital for proper RNA polymerase II functioning, as Parafibromin facilitates histone modification, influencing chromatin structure and gene accessibility. Parafibromin serves as a potential biomarker for diagnosing parathyroid carcinoma, a condition characterized by heterogeneous tumors that can complicate diagnosis. The human CDC73 gene, located on chromosome 1q25, is the homolog of yeast Cdc73 and is implicated in hyperparathyroidism with jaw tumor syndrome (HPT-JT), highlighting Parafibromin′s clinical significance in endocrine tumor suppression.

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Parafibromin Antibody (2H1) References:

1. Familial isolated hyperparathyroidism is rarely caused by germline mutation in HRPT2, the gene for the hyperparathyroidism-jaw tumor syndrome.  |  Simonds, WF., et al. 2004. J Clin Endocrinol Metab. 89: 96-102. PMID: 14715834
2. Hyperparathyroidism-jaw tumor syndrome in Roma families from Portugal is due to a founder mutation of the HRPT2 gene.  |  Cavaco, BM., et al. 2004. J Clin Endocrinol Metab. 89: 1747-52. PMID: 15070940
3. Loss of parafibromin immunoreactivity is a distinguishing feature of parathyroid carcinoma.  |  Tan, MH., et al. 2004. Clin Cancer Res. 10: 6629-37. PMID: 15475453
4. Gene expression of parathyroid tumors: molecular subclassification and identification of the potential malignant phenotype.  |  Haven, CJ., et al. 2004. Cancer Res. 64: 7405-11. PMID: 15492263
5. Genetic analyses of the HRPT2 gene in primary hyperparathyroidism: germline and somatic mutations in familial and sporadic parathyroid tumors.  |  Cetani, F., et al. 2004. J Clin Endocrinol Metab. 89: 5583-91. PMID: 15531515
6. The parafibromin tumor suppressor protein is part of a human Paf1 complex.  |  Rozenblatt-Rosen, O., et al. 2005. Mol Cell Biol. 25: 612-20. PMID: 15632063
7. Nucleolar localization of parafibromin is mediated by three nucleolar localization signals.  |  Hahn, MA. and Marsh, DJ. 2007. FEBS Lett. 581: 5070-4. PMID: 17923126
8. Prognostic role of parafibromin staining and CDC73 mutation in patients with parathyroid carcinoma: A systematic review and meta-analysis based on individual patient data.  |  Zhu, R., et al. 2020. Clin Endocrinol (Oxf). 92: 295-302. PMID: 31945198
9. Parafibromin Is Highly Expressed in Hepatocellular Carcinoma and Its Expression Correlates with Poor Prognosis.  |  Kim, MK. 2022. J Clin Med. 11: PMID: 35407381
10. Diagnostic significance of parafibromin expression in parathyroid carcinoma.  |  Gao, Y., et al. 2022. Hum Pathol. 127: 28-38. PMID: 35654240
11. The roles of the tumor suppressor parafibromin in cancer.  |  Zheng, HC., et al. 2022. Front Cell Dev Biol. 10: 1006400. PMID: 36211470
12. Association Between Parafibromin Expression and Presence of Brown Tumors and Jaw Tumors in Patients with Primary Hyperparathyroidism: Series of Cases with Review of the Literature.  |  Popow, M., et al. 2022. Am J Case Rep. 23: e936135. PMID: 36271606

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Parafibromin Antibody (2H1) has 61 citations in a variety of scientific publications.

Primary antibodies like Anti-Parafibromin Antibody (2H1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Parafibromin Antibody (2H1) Specifications

Alternate Product Name: CDC73

Product Clone Name: 2H1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 87-100

Epitope Target: Parafibromin

Epitope Species: mouse

Product Reactivity/Detection: Parafibromin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 61 citations in various scientific publications

Available Conjugates/Forms for Parafibromin Antibody (2H1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin