Parafibromin Antibody (E-4): sc-373792
- Parafibromin Antibody (E-4) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 253-297 within an internal region of Parafibromin of human origin
- recommended for detection of Parafibromin of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- See Parafibromin (2H1): sc-33638 for Parafibromin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Parafibromin Antibody (E-4) for WB applications. These reagents are now offered in bundles with Parafibromin Antibody (E-4) (see ordering information below).
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Parafibromin Antibody (E-4) is a mouse monoclonal IgG1 antibody that detects Parafibromin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Parafibromin plays a critical role as a tumor suppressor, encoded by the CDC73 gene, which is essential for regulating cell division and maintaining genomic stability. Parafibromin is located in the nucleus, where anti-Parafibromin antibody (E-4) identifies a key component of the RNA polymerase II/Paf1 complex, facilitating the transcriptional regulation of genes by modifying histones. Proper functioning of Parafibromin is vital, as mutations in the CDC73 gene can lead to hyperparathyroidism and parathyroid carcinoma, making Parafibromin an important marker for diagnosing these conditions. Parafibromin interacts with various proteins involved in transcriptional regulation, including the RNA polymerase II large subunit, which underscores Parafibromin′s significance in cellular processes. Parafibromin (E-4) antibody serves as an invaluable tool for researchers studying parathyroid tumors and the underlying mechanisms of endocrine tumorigenesis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Parafibromin Antibody (E-4) References:
- Parafibromin/Hyrax activates Wnt/Wg target gene transcription by direct association with beta-catenin/Armadillo. | Mosimann, C., et al. 2006. Cell. 125: 327-41. PMID: 16630820
- Parafibromin is a nuclear protein with a functional monopartite nuclear localization signal. | Bradley, KJ., et al. 2007. Oncogene. 26: 1213-21. PMID: 16964291
- Parafibromin expression, single-gland involvement, and limited parathyroidectomy in familial isolated hyperparathyroidism. | Iacobone, M., et al. 2007. Surgery. 142: 984-91; discussion 984-91. PMID: 18063086
- Parafibromin, a component of the human PAF complex, regulates growth factors and is required for embryonic development and survival in adult mice. | Wang, P., et al. 2008. Mol Cell Biol. 28: 2930-40. PMID: 18212049
- Parafibromin as a tool for the diagnosis of parathyroid tumors. | Marcocci, C. and Cetani, F. 2008. Adv Anat Pathol. 15: 179; author reply 179-80. PMID: 18434772
- The role of Parafibromin/Hyrax as a nuclear Gli/Ci-interacting protein in Hedgehog target gene control. | Mosimann, C., et al. 2009. Mech Dev. 126: 394-405. PMID: 19368795
- Defective nucleolar localization and dominant interfering properties of a parafibromin L95P missense mutant causing the hyperparathyroidism-jaw tumor syndrome. | Panicker, LM., et al. 2010. Endocr Relat Cancer. 17: 513-24. PMID: 20304979
- Parafibromin and APC as screening markers for malignant potential in atypical parathyroid adenomas. | Juhlin, CC., et al. 2010. Endocr Pathol. 21: 166-77. PMID: 20473645
- Negative parafibromin staining predicts malignant behavior in atypical parathyroid adenomas. | Kruijff, S., et al. 2014. Ann Surg Oncol. 21: 426-33. PMID: 24081804
- POFUT1 promotes gastric cancer progression through Notch/Wnt dual signaling pathways dependent on the parafibromin-NICD1-β-catenin complex. | Dong, S., et al. 2023. J Chin Med Assoc. 86: 806-817. PMID: 37501238
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Parafibromin Antibody (E-4) | sc-373792 | 200 µg/ml | $322.00 | |||
Parafibromin Antibody (E-4): m-IgG Fc BP-HRP Bundle | sc-537873 | 200 µg Ab; 10 µg BP | $361.00 | |||
Parafibromin Antibody (E-4): m-IgGκ BP-HRP Bundle | sc-535311 | 200 µg Ab; 40 µg BP | $361.00 | |||
Parafibromin Antibody (E-4): m-IgG1 BP-HRP Bundle | sc-545420 | 200 µg Ab; 20 µg BP | $361.00 | |||
Parafibromin (E-4) Neutralizing Peptide | sc-373792 P | 100 µg/0.5 ml | $69.00 |