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pan-Cytokeratin Antibody (PK110) - Western Blotting - Image 352390
pan-Cytokeratin Antibody (PK110): sc-53403. Western blot analysis of pan-Cytokeratin expression in Hep G2 whole cell lysate.
pan-Cytokeratin Antibody (PK110): sc-53403. Western blot analysis of pan-Cytokeratin expression in Hep G2 whole cell lysate.

pan-Cytokeratin Antibody (PK110): sc-53403

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Datasheets
  • pan-Cytokeratin Antibody (PK110) is a mouse monoclonal IgG2a κ, cited in 4 publications, provided at 200 µg/ml
  • raised against epidermal keratin of human origin
  • recommended for detection of broad range of Cytokeratin proteins of human origin by WB, IP, IF and IHC(P)
  • See pan-Cytokeratin (C11): sc-8018 for pan-Cytokeratin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for pan-Cytokeratin Antibody (PK110) for WB and IHC(P) applications. These reagents are now offered in bundles with pan-Cytokeratin Antibody (PK110) (see ordering information below).
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Additional Cytokeratin Antibodies including Basic Cytokeratin, Cytokeratin 1, Cytokeratin 10, Cytokeratin 12, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16, Cytokeratin 17, Cytokeratin 18, Cytokeratin 19, Cytokeratin 20, Cytokeratin 23, Cytokeratin 2E, Cytokeratin 2p and Cytokeratin 3

Pan-Cytokeratin Antibody (PK110) is a mouse monoclonal IgG2a antibody that detects pan-Cytokeratin in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Pan-Cytokeratin is a crucial component of the cytoskeleton in epithelial cells, providing structural support and playing a significant role in maintaining cell shape and integrity. Anti-pan-Cytokeratin antibody (PK110) targets proteins primarily located in the cytoplasm of epithelial cells, where pan-Cytokeratin forms a network of intermediate filaments that contribute to the mechanical resilience of tissues. Pan-Cytokeratin expression patterns can help differentiate between various types of epithelial tumors, making pan-Cytokeratin (PK110) monoclonal antibody particularly valuable in cancer diagnostics. For instance, specific cytokeratin profiles can indicate the origin of metastatic tumors, aiding in the determination of appropriate therapeutic strategies. Anti-pan-Cytokeratin antibody (PK110) serves as an invaluable tool for researchers and clinicians seeking to understand epithelial cell biology and its implications in disease.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

pan-Cytokeratin Antibody (PK110) References:

  1. Non-keratinizing undifferentiated carcinoma of the nasopharynx.  |  Boia, ER., et al. 2013. Rom J Morphol Embryol. 54: 839-43. PMID: 24322037
  2. Spontaneous malignant craniopharyngioma in an aged Wistar rat.  |  Heinrichs, M. and Ernst, H. 2016. J Toxicol Pathol. 29: 195-9. PMID: 27559246
  3. Directing adipose-derived stem cells into keratinocyte-like cells: impact of medium composition and culture condition.  |  Petry, L., et al. 2018. J Eur Acad Dermatol Venereol. 32: 2010-2019. PMID: 29705993
  4. Culture of human alveolar epithelial type II cells by sprouting.  |  Khan, P., et al. 2018. Respir Res. 19: 204. PMID: 30340591
  5. Epithelioid sarcoma of the scalp: a case report and literature review.  |  Zhang, XW., et al. 2019. Int J Clin Exp Pathol. 12: 3908-3914. PMID: 31933781
  6. Circulating tumor cells after neoadjuvant chemotherapy are related with recurrence in esophageal squamous cell carcinoma.  |  Ujiie, D., et al. 2021. Esophagus. 18: 566-573. PMID: 33661456
  7. High expression of both desmoplastic stroma and epithelial to mesenchymal transition markers associate with shorter survival in pancreatic ductal adenocarcinoma.  |  Sánchez-Ramírez, D., et al. 2022. Eur J Histochem. 66: PMID: 35174683
  8. Nephrogenic Adenoma of the Prostatic Urethra Mimicking Prostatic and Bladder Carcinomas.  |  Beeter, MC., et al. 2023. Cureus. 15: e35998. PMID: 37041930
  9. Cutaneous Intravascular CD-30-Positive Anaplastic Large Cell Lymphoma: A Case Report and Literature Review.  |  Banafshay, K., et al. 2023. Cureus. 15: e44450. PMID: 37791178
  10. Under the Microscope: A Case Report of Thoracic SMARCA4-Deficient Undifferentiated Tumor with Review of the Literature.  |  Mundada, M., et al. 2024. Turk Patoloji Derg. 40: 128-133. PMID: 38265099

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

pan-Cytokeratin Antibody (PK110)

sc-53403
200 µg/ml
$322.00

pan-Cytokeratin Antibody (PK110): m-IgG Fc BP-HRP Bundle

sc-536957
200 µg Ab; 10 µg BP
$361.00

pan-Cytokeratin Antibody (PK110): m-IgGκ BP-HRP Bundle

sc-534134
200 µg Ab; 40 µg BP
$361.00

Can pan-Cytokeratin (PK110): sc-53403 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, pan-Cytokeratin (PK110): sc-53403 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by ET from WB data in MCF7WB data in MCF7, HeLa, A-431, Hep G2 and T24 whole cell lysates. -SCBT QC
Date published: 2015-03-24
Rated 5 out of 5 by SM from Wonderful for paraffinWonderful for paraffin-embedded human small intestine samples. -SCBT QC
Date published: 2015-03-24
Rated 3 out of 5 by LM from Worked with human transfected lysate by WBWorked with human transfected lysate by WB -SCBT QC
Date published: 2015-02-10
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pan-Cytokeratin Antibody (PK110) is rated 4.0 out of 5 by 3.
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