pan 14-3-3 Antibody (F-1): sc-166061
- pan 14-3-3 Antibody (F-1) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- raised against amino acids 1-246 representing full length 14-3-3 β of human origin
- recommended for detection of pan 14-3-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
- See pan 14-3-3 (B-8): sc-133233 for pan 14-3-3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for pan 14-3-3 Antibody (F-1) for WB and IHC(P) applications. These reagents are now offered in bundles with pan 14-3-3 Antibody (F-1) (see ordering information below).
pan 14-3-3 Antibody (F-1) is a mouse monoclonal IgG2b antibody that detects 14-3-3 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-14-3-3 antibody (F-1) targets the 14-3-3 protein family, which plays a crucial role in regulating numerous cellular processes, particularly in cancer biology, such as apoptosis, mitogenic signaling, and cell-cycle checkpoints. 14-3-3 proteins form dimers that create two binding sites for ligand proteins, facilitating interactions between proteins that might not otherwise associate. This interaction allows for the regulation of various signaling pathways, ensuring proper cellular function and response to external stimuli. 14-3-3 proteins are predominantly located in the cytoplasm, where these proteins can sequester targets, modulate activity, and influence stability through mechanisms such as preventing degradation and facilitating post-translational modifications. 14-3-3 proteins bind to phosphorylated serine/threonine residues, underscoring their importance in cellular signaling, as these modifications are often critical for the activation or inhibition of various pathways. Loss of 14-3-3 expression can lead to dysregulation of these processes, contributing to a range of pathological conditions, including cancer.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
pan 14-3-3 Antibody (F-1) | sc-166061 | 200 µg/ml | $322.00 | |||
pan 14-3-3 Antibody (F-1): m-IgGκ BP-HRP Bundle | sc-534701 | 200 µg Ab; 40 µg BP | $361.00 | |||
pan 14-3-3 Antibody (F-1): m-IgG2b BP-HRP Bundle | sc-549283 | 200 µg Ab; 10 µg BP | $361.00 |