pan 14-3-3 Antibody (B-8): sc-133233

pan 14-3-3 Antibody (B-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects the pan 14-3-3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). pan 14-3-3 (B-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 14-3-3 proteins play a crucial role in regulating numerous cellular processes, particularly those associated with cancer biology, such as apoptosis, mitogenic signaling, and cell-cycle checkpoints. This protein family consists of seven isoforms—14-3-3 β, γ, ε, ζ, η, θ, and Σ—that form dimers, providing two binding sites for ligand proteins. This unique structural feature allows 14-3-3 proteins to facilitate interactions between proteins that might not otherwise associate, which is essential for maintaining cellular homeostasis. The ligands that interact with 14-3-3 proteins typically share a consensus binding motif and are often phosphorylated on serine or threonine residues. By regulating these interactions through mechanisms such as cytoplasmic sequestration, modulation of interaction domains, and prevention of degradation, 14-3-3 proteins significantly influence various signaling pathways. Loss of 14-3-3 expression can lead to a range of pathogenic cellular activities, underscoring 14-3-3′s importance in cellular regulation and disease progression.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-pan 14-3-3 Antibody (B-8) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-pan 14-3-3 Antibody (B-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

pan 14-3-3 Antibody (B-8) Specifications

Alternate Product Name: 14-3-3

Product Clone Name: B-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-246

Epitope Target: 14-3-3 β

Epitope Species: human

Product Reactivity/Detection: pan 14-3-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for pan 14-3-3 Antibody (B-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred pan 14-3-3 antibody is pan 14-3-3 Antibody (H-8): sc-1657.